microRNA-101对子宫颈癌细胞增殖、迁移、凋亡作用的研究
发布时间：2018-11-03 08:47
【摘要】：目的：通过观察高表达的miR-101对子宫颈癌细胞增殖、迁移、凋亡等的影响,并分析靶基因蛋白EZH2和COX-2在子宫颈癌细胞中的表达,探讨miR-101在宫颈细胞癌变中的作用。方法：将人工合成的miR-101的mimics(上调组)和inhibitor(下调组)通过LipofectamineTM2000转染实验组宫颈癌细胞株(Hela, Siha)以及对照组食管鳞癌细胞株Eca109。应用qRT-PCR险测各组细胞中miR-101的表达量、MTT法检测定细胞增殖活力、流式细胞术检测细胞凋亡率、细胞划痕实验观察细胞迁移能力,免疫组织化学法检测EZH2或COX-2蛋白的表达。结果：两种细胞株qRT-PCR结果：上调组miR-101的表达量均高于未转染组,下调组miR-101的表达量均低于未转染组。MTT结果：上调组96后细胞的增殖能力降低,下调组96h后细胞的增殖能力增高。流式细胞结果：Hela,Siha细胞未转染组凋亡率分别为12.2%,8.5%,上调组凋亡率分别为97.6%,76.6%,下调组Siha细胞凋亡率为21.6%。划痕实验结果：Hela上调组的划痕距离(42.65um±2um)低于未转染组(181.38um±2um)。Siha上调组的划痕距离(36.63um±2um)低于未转染组(184.12um±2um)。免疫组织化学结果：上调组EZH2,COX-2蛋白阳性率较未转染组降低,下调组两种蛋白的阳性率较未转染组增高。以上结果差异均具有统计学意义(P0.05)。结论：miR-101的过表达可抑制子宫颈癌细胞增殖、抑制宫颈癌细胞迁移、促进癌细胞的凋亡。miR-101在宫颈细胞癌变过程中发挥重要作用。EZH2,COX-2是miR-101的靶基因之一,二者与miR-101之间呈负性调节关系。
[Abstract]:Aim: to investigate the effects of overexpression of miR-101 on the proliferation, migration and apoptosis of cervical cancer cells, and to analyze the expression of EZH2 and COX-2 in cervical cancer cells, and to explore the role of miR-101 in cervical cancer. Methods: the synthetic miR-101 mimics (up-regulated group) and inhibitor (down-regulated group) were transfected by LipofectamineTM2000 into the cervical cancer cell line (Hela, Siha) and the control esophageal squamous cell carcinoma cell line Eca109.. The expression of miR-101 was measured by qRT-PCR, the proliferative activity was detected by MTT assay, the apoptosis rate was detected by flow cytometry, and the cell migration was observed by cell scratch assay. Immunohistochemical method was used to detect the expression of EZH2 or COX-2 protein. Results: the expression of miR-101 in the up-regulated group was higher than that in the non-transfected group, and the expression of miR-101 in the down-regulated group was lower than that in the untransfected group. MTT results showed that the proliferation ability of the cells in the up-regulated group was decreased after 96 months. The proliferation ability of the down-regulated group was increased after 96 h. Flow cytometry results showed that the apoptotic rate of Hela,Siha cells was 12.28.5in the untransfected group, 97.6and 76.6in the upregulated group, and 21.6in the down-regulated group. The scratch distance (42.65um 卤2um) of Hela up-regulated group was lower than that of 181.38um 卤2um). Siha up-regulated group (36.63um 卤2um) lower than that of untransfected group (184.12um 卤2um). Immunohistochemical results: the positive rate of EZH2,COX-2 protein in up-regulation group was lower than that in non-transfection group, and the positive rate of two proteins in down-regulated group was higher than that in untransfected group. The differences were statistically significant (P0.05). Conclusion: overexpression of miR-101 can inhibit the proliferation of cervical cancer cells, inhibit the migration of cervical cancer cells and promote the apoptosis of cancer cells. MiR-101 plays an important role in the process of cervical cancer. EZH2,COX-2 is one of the target genes of miR-101. There was a negative regulatory relationship between them and miR-101.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.33
本文编号：2307290
[Abstract]:Aim: to investigate the effects of overexpression of miR-101 on the proliferation, migration and apoptosis of cervical cancer cells, and to analyze the expression of EZH2 and COX-2 in cervical cancer cells, and to explore the role of miR-101 in cervical cancer. Methods: the synthetic miR-101 mimics (up-regulated group) and inhibitor (down-regulated group) were transfected by LipofectamineTM2000 into the cervical cancer cell line (Hela, Siha) and the control esophageal squamous cell carcinoma cell line Eca109.. The expression of miR-101 was measured by qRT-PCR, the proliferative activity was detected by MTT assay, the apoptosis rate was detected by flow cytometry, and the cell migration was observed by cell scratch assay. Immunohistochemical method was used to detect the expression of EZH2 or COX-2 protein. Results: the expression of miR-101 in the up-regulated group was higher than that in the non-transfected group, and the expression of miR-101 in the down-regulated group was lower than that in the untransfected group. MTT results showed that the proliferation ability of the cells in the up-regulated group was decreased after 96 months. The proliferation ability of the down-regulated group was increased after 96 h. Flow cytometry results showed that the apoptotic rate of Hela,Siha cells was 12.28.5in the untransfected group, 97.6and 76.6in the upregulated group, and 21.6in the down-regulated group. The scratch distance (42.65um 卤2um) of Hela up-regulated group was lower than that of 181.38um 卤2um). Siha up-regulated group (36.63um 卤2um) lower than that of untransfected group (184.12um 卤2um). Immunohistochemical results: the positive rate of EZH2,COX-2 protein in up-regulation group was lower than that in non-transfection group, and the positive rate of two proteins in down-regulated group was higher than that in untransfected group. The differences were statistically significant (P0.05). Conclusion: overexpression of miR-101 can inhibit the proliferation of cervical cancer cells, inhibit the migration of cervical cancer cells and promote the apoptosis of cancer cells. MiR-101 plays an important role in the process of cervical cancer. EZH2,COX-2 is one of the target genes of miR-101. There was a negative regulatory relationship between them and miR-101.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.33
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