CTHRC1在卵巢癌细胞侵袭转移中的作用及相关机制

发布时间：2018-11-04 08:19

【摘要】：目的·探究胶原三股螺旋重复蛋白1(CTHRC1)在卵巢癌细胞侵袭转移中的作用及其相关机制。方法·采用Western blotting法检测卵巢癌细胞株中CTHRC1蛋白水平。选取高表达细胞株转染以CTHRC1为靶标的sh RNA下调CTHRC1表达水平,以慢病毒lenti-CTHRC1转染低表达细胞株,通过Transwell实验观察细胞过表达或沉默CTHRC1后体外迁移能力和侵袭能力的变化,并检测转移相关蛋白Slug、MMP-2的表达情况。结果·HO8910细胞中CTHRC1表达量较低,lenti-CTHRC1转染后CTHRC1蛋白水平明显升高,细胞迁移和侵袭能力增强,Slug、MMP-2表达显著增高(均P0.05)。Ca OV3细胞则高表达CTHRC1,转染慢病毒lenti-sh CTHRC1后,CTHRC1蛋白水平下调,细胞迁移和侵袭能力明显降低,Slug、MMP-2表达也显著降低(均P0.05)。结论·CTHRC1可能通过上调Slug、MMP-2蛋白表达,进而促进卵巢癌细胞侵袭转移。
[Abstract]:Objective to investigate the role of collagen triple helix repeat protein 1 (CTHRC1) in invasion and metastasis of ovarian cancer cells and its related mechanisms. Methods Western blotting assay was used to detect the level of CTHRC1 protein in ovarian cancer cell line. The expression level of CTHRC1 was down-regulated by sh RNA transfected with CTHRC1 and low expression cell line transfected with lentivirus lenti-CTHRC1. The changes of migration ability and invasiveness after over-expression or silencing of CTHRC1 were observed by Transwell experiment. The expression of Slug,MMP-2 was detected. Results the expression of CTHRC1 in HO8910 cells was low, the level of CTHRC1 protein was significantly increased after lenti-CTHRC1 transfection, the cell migration and invasion ability was enhanced, and the expression of Slug,MMP-2 was significantly increased (P0.05 in). Ca OV3 cells). After transfection of lentivirus lenti-sh CTHRC1, the level of CTHRC1 protein was down-regulated, the cell migration and invasion ability was significantly decreased, and the expression of Slug,MMP-2 was also significantly decreased (P0.05). Conclusion CTHRC1 may promote the invasion and metastasis of ovarian cancer cells by upregulating the expression of Slug,MMP-2 protein.
【作者单位】：上海交通大学医学院附属仁济医院妇产科上海市妇科肿瘤重点实验室;
【基金】：国家自然科学基金(81672564)~~
【分类号】：R737.31

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