EGFR、蛋白激酶B在宫颈癌中表达及EGFR胞外区基因突变的研究

发布时间：2018-11-04 08:36

【摘要】：目的:探讨宫颈癌中表皮生长因子受体(EGFR)、蛋白激酶B(PKB/Akt)的表达及EGFR胞外区基因突变的规律和临床意义,为宫颈癌的发生发展机制和靶向个性化治疗提供实验依据。方法:采用免疫组化SP法检测EGFR、Akt蛋白在16例正常宫颈组织、40例宫颈上皮内瘤变(CIN)组织和60例宫颈癌组织中的表达,并分析其与宫颈癌临床病理的关系。采用酚氯仿法抽提60例宫颈癌组织中DNA,PCR+DNA双向测序法检测EGFR胞外IV亚区基因突变。结果:正常宫颈组、CIN组及宫颈癌组中EGFR蛋白的阳性表达率分别为13.33%、43.33%及76.67%,Akt蛋白的阳性表达率分别为6.25%、35.00%及68.33%,差异有统计学意义(P0.05)。宫颈癌组织中,EGFR表达水平与病理类型、细胞分化程度和临床分期有关(P0.05),但与患者年龄和淋巴结转移无关(P0.05);Akt表达水平与细胞分化程度和临床分期有关(P0.05),但与患者年龄、病理类型和淋巴结转移无关(P0.05)。60例宫颈癌中EGFR基因外显子15突变率为3.33%(2/60),突变类型G→A,未发现外显子14突变。宫颈癌组织中EGFR、Akt表达呈正相关性(r=0.556,P0.05)。结论:EGFR、Akt可作为检测宫颈癌及癌前病变的重要参考指标,也为宫颈癌的多靶点联合治疗提供新思路;宫颈癌中EGFR胞外区可有基因突变,但突变率很低,关于其突变的规律和意义有待进一步研究。
[Abstract]:Objective: to investigate the expression of epidermal growth factor receptor (EGFR), protein kinase B (PKB/Akt) and the mutation of extracellular region of EGFR in cervical carcinoma. To provide experimental basis for the development mechanism of cervical cancer and targeted individualized therapy. Methods: immunohistochemical SP method was used to detect the expression of EGFR,Akt protein in 16 normal cervical tissues, 40 cervical intraepithelial neoplasia (CIN) tissues and 60 cervical carcinoma tissues, and the relationship between the expression of EGFR,Akt protein and the clinicopathology of cervical carcinoma was analyzed. Two way DNA,PCR DNA sequencing method was used to detect the mutation of extracellular IV subregion of EGFR in 60 cases of cervical cancer tissues extracted by phenol chloroform method. Results: the positive expression rates of EGFR protein in normal cervix group, CIN group and cervical carcinoma group were 13.33% and 76.67%, respectively. The positive rates of EGFR protein in normal cervix group, CIN group and cervical cancer group were 6.2535% and 68.33%, respectively. The difference was statistically significant (P0.05). The expression level of EGFR was related to pathological type, cell differentiation and clinical stage (P0.05), but not to age and lymph node metastasis (P0.05). The expression of Akt was correlated with cell differentiation and clinical stage (P0.05), but not with age, pathological type and lymph node metastasis (P0.05). The mutation rate of exon 15 of EGFR gene was 3.33% (2 / 60) in 60 cases of cervical cancer. No mutation in exon 14 was found. There was a positive correlation between the expression of EGFR,Akt in cervical carcinoma (P 0.05). Conclusion: EGFR,Akt can be used as an important reference index for the detection of cervical cancer and precancerous lesions, and it also provides a new idea for multi-target combination therapy of cervical cancer. There may be gene mutations in the extracellular domain of EGFR in cervical cancer, but the mutation rate is very low.
【作者单位】：南昌大学第二附属医院;江西省肿瘤医院;
【基金】：江西省科技厅基金项目(No:20133BBG70069)
【分类号】：R737.33

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