HPV16阳性宫颈鳞癌组织EGFR上游与下游相关蛋白表达临床意义探讨

发布时间：2018-11-06 17:01

【摘要】：目的探讨HPV阳性宫颈鳞癌(cervical squamous cell carcinoma,CSCC)组织中表皮生长因子受体(epidermal growth factor receptor,EGFR)、Rab5a、Rab21、c-jun、c-fos蛋白表达关系及临床意义。方法收集2012-09-01-2013-09-30新疆医科大学附属肿瘤医院病理科保存的初治HPV阳性CSCC组织标本45例。其中维吾尔族人群标本33例,汉族人群标本12例,年龄30~70岁,中位年龄46岁。采用免疫组织化学法检测45例HPV阳性CSCC组织中EGFR、Rab5a、Rab21、c-jun和c-fos蛋白表达情况。结果 EGFR、Rab5a、Rab21、c-jun和c-fos蛋白阳性表达率分别为64.4%、66.7%、60.0%、75.6%和66.7%;EGFR蛋白表达与临床分期(χ2=8.008,P=0.005)、淋巴结转移(χ2=7.162,P=0.007)有关,Rab5a蛋白表达与临床分期(χ2=10.045,P=0.002)、肿瘤分化程度(χ2=3.906,P=0.048)、淋巴结转移(χ2=5.511,P=0.019)有关,Rab21蛋白表达与临床分期(χ2=9.644,P=0.002)、淋巴结转移(χ2=23.766,P0.001)有关,c-jun(χ2=4.022,P=0.045)、c-fos(χ2=18.219,P0.001)蛋白表达与淋巴结转移有关。Spearman等级相关分析显示,c-jun、c-fos蛋白表达与肿瘤分化程度呈负相关(r=-0.347,P=0.020;r=-0.299,P=0.046),与临床分期呈正相关(r=0.362,P=0.015;r=0.329,P=0.028);EGFR与Rab21表达呈负相关(r=-0.322,P=0.031),EGFR与Rab5a、c-jun、c-fos表达呈正相关(r值分别为0.558、0.658和0.755,P值均0.001)。结论 HPV阳性CSCC中Rab21蛋白可能阻碍EGFR介导的信号传导,Rab5a、c-jun、c-fos蛋白可能促进EGFR介导的细胞信号传导,EGFR、c-jun、c-fos、Rab21、Rab5a可能作为CSCC发展、转移的参考指标。联合检测EGFR、c-jun、c-fos、Rab5a、Rab21可为CSCC的诊断、寻找新的治疗靶点和预后评估提供依据。
[Abstract]:Objective to investigate the expression of epidermal growth factor receptor (epidermal growth factor receptor,EGFR) and Rab5a,Rab21,c-jun,c-fos protein in HPV positive cervical squamous cell carcinoma (cervical squamous cell carcinoma,CSCC) and its clinical significance. Methods 45 cases of primary HPV positive CSCC tissue specimens were collected from the Department of Pathology, affiliated Hospital of Xinjiang Medical University, 2012-09-01-2013-09-30. Among them, 33 samples were from Uygur nationality, 12 from Han nationality, aged 30 to 70 years, with a median age of 46 years. The expression of EGFR,Rab5a,Rab21,c-jun and c-fos protein in 45 HPV positive CSCC tissues was detected by immunohistochemical method. Results the positive expression rates of EGFR,Rab5a,Rab21,c-jun and c-fos were 64.4% and 66.7%, respectively. The expression of EGFR protein was correlated with clinical stage (蠂 2 + 8.008) and lymph node metastasis (蠂 2 7. 162% P0. 007). The expression of Rab5a protein was correlated with clinical stage (蠂 2 + 10.045% P0. 002) and the degree of tumor differentiation (蠂 2 + 3. 906% P0. 048). The expression of Rab21 protein was related to the clinical stage (蠂 29.644), lymph node metastasis (蠂 2 + 23.766 P 0.001), c-jun (蠂 2 + 4.022% P < 0.045), c-fos (蠂 2 + 18.219), lymph node metastasis (蠂 2 + 5.511 P 0. 019), the expression of Rab21 protein was associated with clinical stage (蠂 2 9. 64 4 P + 0. 002) and lymph node metastasis (蠂 2 + 23. 76 6 P 0. 001). Spearman grade correlation analysis showed that the expression of c-junn c-fos protein was negatively correlated with the degree of tumor differentiation. There was a positive correlation between the clinical stage and the clinical stage (r = 0.362P = 0.015, P = 0.329P = 0.028). There was a negative correlation between the expression of EGFR and Rab21 (r = 0. 558, P = 0. 755, P = 0. 031, r = 0. 558, P = 0. 755, P = 0.001). Conclusion Rab21 protein in HPV positive CSCC may block EGFR mediated signal transduction, Rab5a,c-jun,c-fos protein may promote EGFR mediated cell signal transduction, EGFR,c-jun,c-fos,Rab21,Rab5a may develop as CSCC. Reference indicators for transfer. Combined detection of EGFR,c-jun,c-fos,Rab5a,Rab21 may provide evidence for the diagnosis of CSCC and for finding new therapeutic targets and prognostic evaluation.
【作者单位】：新疆医科大学附属肿瘤医院放疗中心;新疆医科大学附属肿瘤医病理科院;
【基金】：科技部国际合作项目(2012DFA31560)
【分类号】：R737.33

【参考文献】