Prdx4蛋白表达水平改变对人宫颈癌HeLa细胞增殖和凋亡的影响

发布时间：2018-11-11 16:05

【摘要】：目的 :观察过氧化物还原酶4(peroxiredoxin 4,Prdx4)蛋白表达水平的改变对人宫颈癌He La细胞增殖和凋亡的影响。方法:采用脂质体瞬时转染法将表达质粒pc DNA3.0-HA-Prdx4(以pc DNA3.0-HA空载体为阴性对照)和针对人Prdx 4基因的3种小干扰RNA(small interference RNA,si RNA)(以阴性对照si RNA为阴性对照)分别转染宫颈癌He La细胞;用蛋白质印迹法证实Prdx4蛋白表达水平被改变后,采用MTT法和FCM法分别检测He La细胞的增殖活性和凋亡情况。结果 :pc DNA3.0-HA-Prdx4质粒转染组He La细胞的Prdx4蛋白表达水平明显上调(P0.05),而3个Prdx4 si RNA干扰组的Prdx4表达水平均明显下调(P值均0.05)。Prdx4过表达组He La细胞的增殖能力与空白对照组及空载体转染组比较,未发生明显改变(P值均0.05),且凋亡细胞百分率差异也无统计学意义(P值均0.05);而3个Prdx4表达干扰组He La细胞的增殖能力较阴性对照组均明显降低(P值均0.05),凋亡细胞百分率均明显提高(P值均0.05)。结论 :Prdx4蛋白表达水平上调对He La细胞的增殖和凋亡没有明显影响,但其下调会抑制He La细胞增殖并促进细胞凋亡。推测Prdx4有可能成为临床治疗宫颈癌的一个潜在靶点。
[Abstract]:Aim: to observe the effect of the expression level of peroxiredoxin _ 4 and Prdx4 on the proliferation and apoptosis of human cervical cancer He La cells. Methods: the expression plasmid pc DNA3.0-HA-Prdx4 (pc DNA3.0-HA empty vector as negative control) and three kinds of small interfering RNA (small interference RNA, targeting human Prdx 4 gene were transfected by liposome transient transfection method. Si RNA) (was transfected into cervical cancer He La cells with negative control si RNA as negative control. After the expression of Prdx4 protein was confirmed by Western blotting, the proliferative activity and apoptosis of He La cells were detected by MTT assay and FCM assay, respectively. Results: the expression of Prdx4 protein was up-regulated in He La cells transfected with pc DNA3.0-HA-Prdx4 plasmid (P0.05). The expression of Prdx4 in the three Prdx4 si RNA interference groups was significantly down-regulated (P < 0. 05). The proliferative ability of He La cells in the Prdx4 overexpression group was not significantly different from that in the blank control group and the empty vector transfection group (P < 0. 05). There was no significant difference in the percentage of apoptotic cells (P < 0.05). The proliferative ability of He La cells in the three Prdx4 interference groups was significantly lower than that in the negative control group (P < 0. 05), and the percentage of apoptotic cells was significantly increased (P < 0. 05). Conclusion: the up-regulation of Prdx4 protein expression has no significant effect on the proliferation and apoptosis of He La cells, but its down-regulation can inhibit the proliferation of He La cells and promote apoptosis. It is speculated that Prdx4 may be a potential target for clinical treatment of cervical cancer.
【作者单位】：南方医科大学病理生理学教研室广东省功能蛋白质组学重点实验室;
【基金】：国家自然科学基金资助项目(编号:81171958)~~
【分类号】：R737.33

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