经γ射线辐照后的脂肪间充质干细胞对大鼠薄型子宫内膜的治疗作用

发布时间：2018-11-12 16:43

【摘要】：目的直接进行脂肪间充质干细胞(ADSCs)移植治疗疾病,有可能因为干细胞的无限增殖能力导致治疗的风险性升高,尤其是有致瘤的风险。本实验将γ射线辐照后的ADSCs 移植入小鼠薄型子宫内膜模型,以提高治疗安全性。方法 ADSCs取材于雌性SD大鼠,应用流式细胞技术进行鉴定后,分别以0、5 和10 Gy 的γ射线辐照ADSCs。24 只薄型子宫内膜模型SD大鼠随机分为4 组,在造模6-8 h 后子宫内移植未经辐照的ADSCs(组Ⅰ)、经5 Gy辐照的ADSCs(组Ⅱ)、经10 Gy辐照的ADSCs(组Ⅲ),或仅子宫内注射PBS(组Ⅳ)。测量各组大鼠细胞移植后子宫内膜的厚度变化以观察治疗效果。结果 ADSCs经10 Gy的辐照后完全失去了增殖能力。组Ⅰ和组Ⅱ大鼠在细胞移植治疗后子宫内膜厚度比组Ⅳ有显著增加(P0.01),但是组Ⅲ和组Ⅳ大鼠的子宫内膜厚度无显著差异。结论γ射线破坏了ADSCs的增殖能力,经10 Gy照射后,ADSCs完全失去了增殖能力并失去了治疗能力。经5 Gy照射后的ADSCs部分丧失了增殖能力,但仍有治疗效果,能够改善子宫内膜的厚度。
[Abstract]:Objective to treat diseases by direct transplantation of adipose mesenchymal stem cells (ADSCs), it is possible that the unlimited proliferation of stem cells may lead to increased risk of treatment, especially the risk of tumorigenesis. In this study, 纬-ray irradiated ADSCs was transplanted into mouse model of thin endometrium to improve the safety of treatment. Methods ADSCs was obtained from female SD rats and identified by flow cytometry. The ADSCs.24 model SD rats were randomly divided into 4 groups, which were irradiated with 纬 -rays of 0 ~ 5 and 10 Gy for 5 Gy and 10 Gy, respectively. ADSCs (group 鈪，

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