JQ1对宫颈癌HeLa细胞增殖的影响及机制研究
发布时间：2018-11-18 21:26
【摘要】：目的:观察JQ1对宫颈癌HeLa细胞增殖的影响,探讨JQ1对宫颈癌HeLa细胞增殖的调控作用及其机制,为临床治疗宫颈癌提供新的治疗途径。方法:1.体外培养HeLa细胞,按每孔细胞0.5×104个接种至96孔板,细胞贴壁100min后,将细胞分成二甲亚砜(DMSO)对照组和JQ1处理组(终浓度分别为0.01、0.1、1和10μmol/L),细胞处理96h,采用MTT比色法检测JQ1对HeLa细胞增殖的影响;2.体外培养HeLa细胞,按每孔细胞500个接种至6孔板,细胞贴壁100min后,将细胞分成DMSO对照组和JQ1处理组(终浓度分别为0.02、0.2、2和20μmol/L),细胞处理12 d,计算细胞克隆形成数量变化;3.体外培养HeLa细胞,将细胞分成DMSO对照组和JQ1处理组(终浓度分别为0.01、0.1、1和10μmol/L),收集细胞RNA和蛋白,采用实时荧光定量PCR和Westernblot检测细胞中c-Myc表达变化。结果:1.与DMSO对照组相比,JQ1抑制了HeLa细胞的增殖,且成剂量依赖性,0.01、0.1、1和10μmol/L JQ1处理组细胞增殖分别下降至0.8±0.02、0.71±0.1、0.61±0.01和0.45±0.02,均P0.05;2.与DMSO对照组相比,0.01、0.1、1和10μmol/L JQ1处理组均抑制了HeLa细胞克隆形成(细胞克隆形成数量:276.7±12比156±11、111.7±10、80.3±6、4.3±2,均P0.05);3.与DMSO对照组相比,0.01、0.1、1和10μmol/L JQ1处理组抑制了HeLa细胞c-Myc m RNA(1.01±0.01倍比0.74±0.02倍、0.63±0.021倍、0.52±0.01倍、0.45±0.02倍,均P0.05)和蛋白表达(1.02±0.01倍比0.64±0.15倍、0.53±0.02倍、0.42±0.11倍、0.33±0.02倍,均P0.05)。结论:JQ1抑制了HeLa细胞增殖,其可能的机制是通过抑制c-Myc的表达。
[Abstract]:Aim: to observe the effect of JQ1 on the proliferation of cervical cancer HeLa cells, and to explore the regulatory effect and mechanism of JQ1 on the proliferation of cervical cancer HeLa cells, so as to provide a new therapeutic approach for clinical treatment of cervical cancer. Methods: 1. HeLa cells were cultured in vitro. The cells were inoculated to 96 well plates according to 0.5 脳 104 cells per well. The cells were divided into dimethyl sulfoxide (DMSO) control group and JQ1 treated group (0. 01 渭 mol/L and 0. 01 渭 mol/L, respectively). The cells were treated for 96 h. The effect of JQ1 on the proliferation of HeLa cells was detected by MTT colorimetry. 2. HeLa cells were cultured in vitro. The cells were inoculated to 6 well plates according to 500 cells per well. After the cells adhered to 100min, the cells were divided into two groups: DMSO control group and JQ1 treatment group (the final concentration was 0.02 渭 mol/L and 20 渭 mol/L, respectively), and the cells were treated for 12 days. The number of cell clone formation was calculated. 3. HeLa cells were cultured in vitro. The cells were divided into DMSO control group and JQ1 treated group (the final concentration was 0.01 渭 mol/L and 10 渭 mol/L, respectively). The cell RNA and protein were collected and the changes of c-Myc expression in the cells were detected by real-time fluorescence quantitative PCR and Westernblot. The result is 1: 1. Compared with DMSO control group, JQ1 inhibited the proliferation of HeLa cells in a dose-dependent manner. The proliferation of HeLa cells in the 0. 01 渭 mol/L JQ1 and 10 渭 mol/L JQ1 groups decreased to 0. 8 卤0. 02 卤0. 71 卤0. 01 卤0. 61 卤0. 01 and 0. 45 卤0. 02, respectively (P 0. 05). Compared with DMSO control group, both 0. 01 渭 mol/L JQ1 and 10 渭 mol/L JQ1 treatment group inhibited HeLa cell clone formation (276.7 卤12 vs 156 卤11111.7 卤10 0. 3 卤64.3 卤2, P0.05). Compared with the control group of DMSO, the c-Myc m RNA (of HeLa cells was inhibited by 0.01 卤0.01,0.63 卤0.021, 0.52 卤0.01,0.45 卤0.02,0.74 卤0.02,0.63 卤0.021, 0.52 卤0.01,0.45 卤0.02 times respectively in the 0. 01 卤0. 01 and 10 渭 mol/L JQ1 groups. Protein expression (1.02 卤0.01 times vs 0.64 卤0.15 times, 0.53 卤0.02 fold, 0.42 卤0.11 fold, 0.33 卤0.02 fold, P0.05). Conclusion: JQ1 inhibits the proliferation of HeLa cells by inhibiting the expression of c-Myc.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R737.33
本文编号：2341301
[Abstract]:Aim: to observe the effect of JQ1 on the proliferation of cervical cancer HeLa cells, and to explore the regulatory effect and mechanism of JQ1 on the proliferation of cervical cancer HeLa cells, so as to provide a new therapeutic approach for clinical treatment of cervical cancer. Methods: 1. HeLa cells were cultured in vitro. The cells were inoculated to 96 well plates according to 0.5 脳 104 cells per well. The cells were divided into dimethyl sulfoxide (DMSO) control group and JQ1 treated group (0. 01 渭 mol/L and 0. 01 渭 mol/L, respectively). The cells were treated for 96 h. The effect of JQ1 on the proliferation of HeLa cells was detected by MTT colorimetry. 2. HeLa cells were cultured in vitro. The cells were inoculated to 6 well plates according to 500 cells per well. After the cells adhered to 100min, the cells were divided into two groups: DMSO control group and JQ1 treatment group (the final concentration was 0.02 渭 mol/L and 20 渭 mol/L, respectively), and the cells were treated for 12 days. The number of cell clone formation was calculated. 3. HeLa cells were cultured in vitro. The cells were divided into DMSO control group and JQ1 treated group (the final concentration was 0.01 渭 mol/L and 10 渭 mol/L, respectively). The cell RNA and protein were collected and the changes of c-Myc expression in the cells were detected by real-time fluorescence quantitative PCR and Westernblot. The result is 1: 1. Compared with DMSO control group, JQ1 inhibited the proliferation of HeLa cells in a dose-dependent manner. The proliferation of HeLa cells in the 0. 01 渭 mol/L JQ1 and 10 渭 mol/L JQ1 groups decreased to 0. 8 卤0. 02 卤0. 71 卤0. 01 卤0. 61 卤0. 01 and 0. 45 卤0. 02, respectively (P 0. 05). Compared with DMSO control group, both 0. 01 渭 mol/L JQ1 and 10 渭 mol/L JQ1 treatment group inhibited HeLa cell clone formation (276.7 卤12 vs 156 卤11111.7 卤10 0. 3 卤64.3 卤2, P0.05). Compared with the control group of DMSO, the c-Myc m RNA (of HeLa cells was inhibited by 0.01 卤0.01,0.63 卤0.021, 0.52 卤0.01,0.45 卤0.02,0.74 卤0.02,0.63 卤0.021, 0.52 卤0.01,0.45 卤0.02 times respectively in the 0. 01 卤0. 01 and 10 渭 mol/L JQ1 groups. Protein expression (1.02 卤0.01 times vs 0.64 卤0.15 times, 0.53 卤0.02 fold, 0.42 卤0.11 fold, 0.33 卤0.02 fold, P0.05). Conclusion: JQ1 inhibits the proliferation of HeLa cells by inhibiting the expression of c-Myc.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R737.33
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相关期刊论文 前2条
1 刘桂玲;岳瑛;;原癌基因c-myc与宫颈癌的研究进展[J];中国老年学杂志;2013年09期
2 薛翔;公丕军;范引侠;;HeLa和SiHa细胞中Skp2、p27和C-myc的表达[J];实用妇产科杂志;2012年08期
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