OSTP-DDP靶向抑制卵巢癌A2780细胞生长作用的研究
发布时间：2018-11-21 15:53
【摘要】：目的研究卵巢癌特异性结合肽(ovarian cancer specific targeting peptide,OSTP)与顺铂(cis-dichlorodiamineplatinum,DDP)偶联物(OSTP-DDP)对卵巢癌A2780细胞的靶向抑制作用。方法化学合成OSTP-DDP,体外培养卵巢癌A2780细胞,采用CCK-8法分别检测OSTP-DDP和DDP对卵巢癌A2780细胞的生长抑制作用;通过Annexin V-FITC凋亡试剂盒分别检测OSTP-DDP和DDP对卵巢癌A2780细胞的周期和凋亡作用。结果通过质谱分析和高效液相色谱(HPLC)分析,提示成功合成OSTP-DDP。CCK-8法检测显示,OSTP-DDP与DDP分别以不同浓度(10、20、40、80、160、320μmol·L~(-1))处理卵巢癌A2780细胞24、48、72 h后,均可对该细胞的生长起到抑制作用,且呈时间、浓度依赖性。且OSTP-DDP的作用强于DDP(P0.05),提示OSTP-DDP具有靶向抑制细胞生长作用。流式细胞术检测结果显示,经OSTP-DDP和DDP处理后,细胞周期均被阻滞于G_1期,作用72 h后,OSTP-DDP对卵巢癌A2780细胞的周期抑制作用强于DDP,差异具有统计学意义(P0.05)。作用24、48、72 h后,OSTP-DDP对卵巢癌A2780细胞凋亡的作用强于DDP(P0.01)差异具有统计学意义,提示OSTP-DDP具有较强的靶向诱导细胞凋亡作用。结论 OSTP-DDP对卵巢癌A2780细胞的生长具有靶向抑制作用,OSTP作为化疗药物靶向载体治疗卵巢癌有良好的应用前景。
[Abstract]:Objective to investigate the targeting inhibition of ovarian cancer A2780 cells by cisplatin (cis-dichlorodiamineplatinum,DDP) conjugate (OSTP-DDP) and ovarian cancer specific binding peptide (ovarian cancer specific targeting peptide,OSTP). Methods Ovarian cancer A2780 cells were cultured by chemically synthesized OSTP-DDP, and the growth inhibitory effects of OSTP-DDP and DDP on A2780 cells were detected by CCK-8 assay. The effects of OSTP-DDP and DDP on cell cycle and apoptosis of ovarian cancer A2780 cells were detected by Annexin V-FITC apoptosis kit. Results the results of mass spectrometry and high performance liquid chromatography (HPLC) showed that the successful synthetic OSTP-DDP.CCK-8 assay showed that OSTP-DDP and DDP were treated with different concentration (10 ~ 20 ~ 40 ~ (80) 160320 渭 mol / L ~ (-1) for 72 h, respectively. The growth of the cells was inhibited in a time and concentration dependent manner. The effect of OSTP-DDP was stronger than that of DDP (P 0.05), suggesting that OSTP-DDP had a targeted inhibitory effect on cell growth. The results of flow cytometry showed that after OSTP-DDP and DDP treatment, the cell cycle was blocked in G1 phase. After 72 h of treatment, the inhibitory effect of OSTP-DDP on A2780 cell cycle of ovarian cancer was stronger than that of DDP,. The difference was statistically significant (P0.05). The effect of OSTP-DDP on apoptosis of ovarian cancer A2780 cells was stronger than that of DDP (P0.01) after 72 h treatment, suggesting that OSTP-DDP had a strong targeting effect on inducing apoptosis of A2780 cells. Conclusion OSTP-DDP has a targeted inhibitory effect on the growth of ovarian cancer A2780 cells. OSTP as a targeted carrier of chemotherapeutic drugs has a good prospect in the treatment of ovarian cancer.
【作者单位】： 南华大学肿瘤研究所;南华大学附属第一医院临床研究所;
【基金】：国家自然科学基金资助项目(No 81472449,81101988)
【分类号】：R737.31
，
本文编号：2347431
[Abstract]:Objective to investigate the targeting inhibition of ovarian cancer A2780 cells by cisplatin (cis-dichlorodiamineplatinum,DDP) conjugate (OSTP-DDP) and ovarian cancer specific binding peptide (ovarian cancer specific targeting peptide,OSTP). Methods Ovarian cancer A2780 cells were cultured by chemically synthesized OSTP-DDP, and the growth inhibitory effects of OSTP-DDP and DDP on A2780 cells were detected by CCK-8 assay. The effects of OSTP-DDP and DDP on cell cycle and apoptosis of ovarian cancer A2780 cells were detected by Annexin V-FITC apoptosis kit. Results the results of mass spectrometry and high performance liquid chromatography (HPLC) showed that the successful synthetic OSTP-DDP.CCK-8 assay showed that OSTP-DDP and DDP were treated with different concentration (10 ~ 20 ~ 40 ~ (80) 160320 渭 mol / L ~ (-1) for 72 h, respectively. The growth of the cells was inhibited in a time and concentration dependent manner. The effect of OSTP-DDP was stronger than that of DDP (P 0.05), suggesting that OSTP-DDP had a targeted inhibitory effect on cell growth. The results of flow cytometry showed that after OSTP-DDP and DDP treatment, the cell cycle was blocked in G1 phase. After 72 h of treatment, the inhibitory effect of OSTP-DDP on A2780 cell cycle of ovarian cancer was stronger than that of DDP,. The difference was statistically significant (P0.05). The effect of OSTP-DDP on apoptosis of ovarian cancer A2780 cells was stronger than that of DDP (P0.01) after 72 h treatment, suggesting that OSTP-DDP had a strong targeting effect on inducing apoptosis of A2780 cells. Conclusion OSTP-DDP has a targeted inhibitory effect on the growth of ovarian cancer A2780 cells. OSTP as a targeted carrier of chemotherapeutic drugs has a good prospect in the treatment of ovarian cancer.
【作者单位】： 南华大学肿瘤研究所;南华大学附属第一医院临床研究所;
【基金】：国家自然科学基金资助项目(No 81472449,81101988)
【分类号】：R737.31
，
本文编号：2347431
本文链接：https://www.wllwen.com/yixuelunwen/fuchankeerkelunwen/2347431.html
最近更新
教材专著
