人脐带间充质干细胞移植对围绝经期大鼠INHB和AMH的影响

发布时间：2018-11-23 12:14

【摘要】：目的:探讨人脐带间充质干细胞(h UCMSCs)移植对围绝经期大鼠AMH、INHB的影响。方法:阴道脱落细胞涂片筛选30只13~15月龄的SPF级SD围绝经期大鼠模型,随机分为A、B、C组(每组10只)。A组不予处理;B组经尾静脉移植浓度1×106细胞/ml h UCMSCs 1ml,隔日1次,共2次;C组移植等量的细胞培养液。于成模时和末次移植后7天和14天,检测各组大鼠的AMH、INHB水平。结果:3组成模时及末次移植后7天的AMH、INHB水平比较,差异均无统计学意义(P0.05)。末次移植后14天,B组的AMH、INHB水平高于A、C组,差异有统计学意义(P0.05);移植后,A、C组的AMH、INHB水平逐渐下降,移植后第14天时下降显著,与成模时比较差异有统计学意义(P0.05)。结论:h UCMSCs移植可延缓围绝经期SD大鼠AMH、INHB下降水平,提示对延缓围绝经期大鼠卵巢储备功能下降有积极意义。
[Abstract]:Aim: to investigate the effect of human umbilical cord mesenchymal stem cell (h UCMSCs) transplantation on AMH,INHB in perimenopausal rats. Methods: 30 SPF grade SD peri-menopausal rats of 13 and 15 months old were screened by vaginal exfoliative cell smear and randomly divided into two groups: group A (n = 10),). A group (n = 10) and control group (n = 10). The concentration of 1 脳 10 ~ 6 cells / ml h UCMSCs was 1 脳 10 ~ 6 cells / ml h UCMSCs 1 ml in group B, 2 times every other day, and the same amount of cell culture medium was transplanted in group C. The levels of AMH,INHB were measured at the time of model formation and 7 and 14 days after the last transplantation. Results: (3) there was no significant difference in AMH,INHB levels at the time of model formation and 7 days after the last transplantation (P0.05). On the 14th day after the last transplantation, the level of AMH,INHB in group B was higher than that in group A (P 0.05). After transplantation, the level of AMH,INHB in group A C decreased gradually, and decreased significantly on the 14th day after transplantation, which was significantly different from the time of model formation (P0.05). Conclusion: h UCMSCs transplantation can delay the decrease of AMH,INHB in peri-menopausal SD rats, suggesting that it has positive significance to delay the decline of ovarian reserve function in perimenopausal rats.
【作者单位】：广东医学院教学医院广东省第二人民医院;
【分类号】：R711.75

【参考文献】