雌激素对子宫内膜癌细胞内钙离子水平的调控的研究

发布时间：2018-11-24 13:29

【摘要】：目的:研究雌激素通过子宫内膜癌细胞膜受体非基因效应对细胞内钙离子的调控。方法:17β-雌二醇(E2)和17β-雌二醇偶联牛血清白蛋白(E2-BSA)作用于子宫内膜癌Ishikawa细胞系,在细胞外液分别为细胞培养基、Tyrode's液和磷酸缓冲液(PBS)的条件下,激光共聚焦显微镜检测雌激素作用后子宫内膜癌细胞胞浆钙离子的浓度变化。结果:细胞外液为培养基时,10nmol/L E2和E2-BSA作用100s内即引起钙离子浓度的增高,E2表现为单峰,E2-BSA表现为双峰;细胞外液为Tyrode's液时,E2作用后钙离子浓度无改变,E2-BSA引起钙离子浓度快速增高,100s后回落;细胞外液为PBS时,E2作用后钙离子浓度瞬时下降,E2-BSA作用后钙离子浓度快速下降,50s后趋于稳定。结论:雌激素可调节子宫内膜癌细胞内钙离子浓度,细胞外液可影响其调控作用。
[Abstract]:Aim: to study the effect of estrogen on intracellular calcium in endometrial carcinoma. Methods: 17 尾 -estradiol (E2) and 17 尾 -estradiol combined with bovine serum albumin (E2-BSA) were used in the treatment of endometrial carcinoma Ishikawa cell line. The extracellular solution was cell culture medium, Tyrode's solution and phosphoric acid buffer solution (PBS), respectively. The changes of Ca ~ (2 +) in endometrial cancer cells after estrogen treatment were detected by confocal laser microscopy. Results: when extracellular fluid was used as culture medium, 10nmol/L E2 and E2-BSA increased calcium concentration within 100 seconds, E2 showed single peak and E2-BSA showed double peak. When the extracellular solution was Tyrode's solution, the concentration of Ca ~ (2 +) did not change after the action of E2, but the concentration of Ca ~ (2 +) was increased rapidly by E2-BSA, and then decreased after 100 s. When the extracellular fluid was PBS, the concentration of Ca ~ (2 +) decreased instantaneously after the action of E _ 2, the concentration of Ca ~ (2 +) decreased rapidly after the action of E2-BSA, and tended to be stable after 50 s. Conclusion: estrogen can regulate the concentration of calcium in endometrial cancer cells, and extracellular fluid can influence its regulation.
【作者单位】：北京积水潭医院妇产科;北京大学人民医院妇产科;
【分类号】：R737.33

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