TLR4炎症通路中TLR4乙酰化作用在妊娠期糖尿病发病机制中的研究

发布时间：2018-11-24 21:00

【摘要】：目的:研究Toll样变体4(Toll-like receptor 4,TLR4)乙酰化在妊娠期糖尿病(GDM)孕妇外周血单核细胞中的表达及其在TLR4炎症通路中的作用,探讨GDM的炎症发病机制。方法:取正常孕妇、GDM孕妇各30例,提取外周血单核细胞进行体外脂多糖(lipopolysaccharide,LPS)干预培养。免疫共沉淀法、Western blot法检测TLR4乙酰化的表达;ELISA法检测炎症因子TNF-α、IL-1、IL-10的水平。结果:GDM组发生TLR4乙酰化变化,而对照组未检测到TLR4乙酰化;LPS干预后,TLR4乙酰化的程度明显增加,且GDM+LPS组TLR4乙酰化程度明显高于GDM组和正常+LPS组(P0.05)。4组间炎症因子TNF-α、IL-1、IL-10水平比较差异均有统计学意义(P0.05)。结论 :GDM孕妇体内单核细胞存在TLR4乙酰化并影响TLR4炎症通路的活化介导炎症因子的释放,促进孕妇体内抗炎-致炎的失衡,从而参与GDM的发生。
[Abstract]:Aim: to investigate the expression of Toll like variant 4 (Toll-like receptor 4 TLR4) acetylation in peripheral blood monocytes of pregnant women with gestational diabetes mellitus (GDM) and its role in the TLR4 inflammatory pathway, and to explore the pathogenesis of GDM inflammation. Methods: peripheral blood monocytes were extracted from 30 normal pregnant women and 30 GDM pregnant women and cultured in vitro with lipopolysaccharide (lipopolysaccharide,LPS). The expression of TLR4 acetylation was detected by, Western blot and the levels of TNF- 伪 and IL-1,IL-10 by ELISA. Results: TLR4 acetylation occurred in GDM group, but TLR4 acetylation was not detected in control group. After the intervention of LPS, the degree of acetylation of TLR4 increased significantly, and the degree of acetylation of TLR4 in GDM LPS group was significantly higher than that in GDM group and normal LPS group (P0.05). The inflammatory cytokines TNF- 伪, IL-1, in GDM LPS group were significantly higher than those in GDM group and normal LPS group (P0.05). IL-10 levels were significantly different (P0.05). Conclusion: there is TLR4 acetylation in monocytes of pregnant women with GDM, which affects the activation of TLR4 inflammatory pathway and mediates the release of inflammatory factors, promotes the imbalance of anti-inflammatory and inflammation in pregnant women, and thus participates in the occurrence of GDM.
【作者单位】：安徽医科大学第一附属医院妇产科产前诊断中心;
【基金】：安徽省自然科学基金项目(编号:1208085MH172)
【分类号】：R714.256

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