子宫颈癌中高危型HPV感染与c-jun和c-fos蛋白表达的关系
发布时间：2018-11-29 08:48
【摘要】：目的探讨子宫颈癌中高危型人乳头状瘤病毒(high-risk human papilloma virus,HR-HPV)感染与c-jun和c-fos蛋白表达的关系及临床意义。方法采用Cervista技术对70例子宫颈鳞状细胞癌(cervical squmaous cell carcinoma,CSCC)、60例子宫颈上皮内病变(cervical intraepithelial neoplasia,CIN)及20例慢性子宫颈炎行HR-HPV检测,同时应用免疫组化SP法检测AP-1通路重要组份c-jun和c-fos蛋白的表达,分析HR-HPV感染与c-jun和c-fos蛋白表达的相关性及与子宫颈癌临床病理特征的关系。结果 70例CSCC中69例HR-HPV呈阳性,阳性率98.6%(69/70),其中A9组阳性占比最高,达85.5%(59/69)。70例CSCC中c-jun和c-fos的阳性率分别为80%(56/70)、85.7%(60/70),30例CIN2+CIN3中的阳性率均为70%(21/30),30例CIN1中的阳性率分别为20%(6/30)、23.3%(7/30),20例慢性子宫颈炎中的阳性率均为0。CSCC组与CIN组及慢性子宫颈炎组相比差异有统计学意义(P0.05)。c-jun、c-fos阳性率在CSCC的临床分期、组织学分级等分组中差异有统计学意义(P0.05);CSCC中HR-HPV阳性与c-jun、c-fos的表达均呈正相关(P0.01)。结论 CSCC中HR-HPV感染具有显著的亚型特点,且与c-jun和c-fos表达呈正相关,提示HR-HPV感染后AP-1通路活化可能与肿瘤的发生、发展相关。
[Abstract]:Objective to investigate the relationship between high risk human papillomavirus (high-risk human papilloma virus,HR-HPV) infection and the expression of c-jun and c-fos protein in cervical carcinoma and its clinical significance. Methods 70 cases of cervical squamous cell carcinoma (cervical squmaous cell carcinoma,CSCC), 60 cases of cervical intraepithelial lesion (cervical intraepithelial neoplasia,CIN) and 20 cases of chronic cervicitis were detected by Cervista. The expression of c-jun and c-fos proteins in AP-1 pathway was detected by immunohistochemical SP method. The relationship between HR-HPV infection and c-jun and c-fos expression and clinicopathological features of cervical cancer was analyzed. Results 69 cases of CSCC were positive for HR-HPV, the positive rate was 98.6% (69 / 70), the positive rate of A9 group was the highest (85.5% (59 / 69). The positive rates of c-jun and c-fos in 70 cases of CSCC were 80% (56 / 70), respectively. 85.7% (60 / 70), 30 cases of CIN2 CIN3 were 70% (21 / 30), 30 cases of CIN1 were 20% (6 / 30), 23. 3% (7 / 30), respectively. In 20 cases of chronic cervicitis, the positive rate of c-junc-fos in 0.CSCC group was significantly higher than that in CIN group and chronic cervicitis group (P0.05), and the positive rate of c-junc-fos in CSCC was significantly higher than that in CIN group and chronic cervicitis group (P0.05). The difference in histological grading was statistically significant (P0.05). There was a positive correlation between the expression of HR-HPV and c-fos in CSCC (P0.01). Conclusion there is a significant subtype of HR-HPV infection in CSCC, and it is positively correlated with the expression of c-jun and c-fos, suggesting that the activation of AP-1 pathway after HR-HPV infection may be related to the occurrence and development of tumor.
【作者单位】： 江西南昌解放军第九四医院病理科;
【基金】：南京军区医药卫生资助(12MB022)
【分类号】：R737.33
，
本文编号：2364542
[Abstract]:Objective to investigate the relationship between high risk human papillomavirus (high-risk human papilloma virus,HR-HPV) infection and the expression of c-jun and c-fos protein in cervical carcinoma and its clinical significance. Methods 70 cases of cervical squamous cell carcinoma (cervical squmaous cell carcinoma,CSCC), 60 cases of cervical intraepithelial lesion (cervical intraepithelial neoplasia,CIN) and 20 cases of chronic cervicitis were detected by Cervista. The expression of c-jun and c-fos proteins in AP-1 pathway was detected by immunohistochemical SP method. The relationship between HR-HPV infection and c-jun and c-fos expression and clinicopathological features of cervical cancer was analyzed. Results 69 cases of CSCC were positive for HR-HPV, the positive rate was 98.6% (69 / 70), the positive rate of A9 group was the highest (85.5% (59 / 69). The positive rates of c-jun and c-fos in 70 cases of CSCC were 80% (56 / 70), respectively. 85.7% (60 / 70), 30 cases of CIN2 CIN3 were 70% (21 / 30), 30 cases of CIN1 were 20% (6 / 30), 23. 3% (7 / 30), respectively. In 20 cases of chronic cervicitis, the positive rate of c-junc-fos in 0.CSCC group was significantly higher than that in CIN group and chronic cervicitis group (P0.05), and the positive rate of c-junc-fos in CSCC was significantly higher than that in CIN group and chronic cervicitis group (P0.05). The difference in histological grading was statistically significant (P0.05). There was a positive correlation between the expression of HR-HPV and c-fos in CSCC (P0.01). Conclusion there is a significant subtype of HR-HPV infection in CSCC, and it is positively correlated with the expression of c-jun and c-fos, suggesting that the activation of AP-1 pathway after HR-HPV infection may be related to the occurrence and development of tumor.
【作者单位】： 江西南昌解放军第九四医院病理科;
【基金】：南京军区医药卫生资助(12MB022)
【分类号】：R737.33
，
本文编号：2364542
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