维、汉族HPV阳性宫颈液基细胞学中L1蛋白的表达

发布时间：2018-12-14 13:56

【摘要】：目的：探讨HPV L1蛋白在新疆维、汉两个民族妇女HPV阳性的宫颈液基细胞学标本中的表达、意义及差异,为新疆地区妇女宫颈病变的防治工作提供理论依据。方法：选取2011年12月至2012年10月就诊于新疆维吾尔自治区人民医院维、汉族女性患者182名,运用免疫组化SP法检测Ll蛋白在HPV阳性的宫颈液基细胞学样本中的表达。结果：85名维吾尔族患者中L1的表达阳性率为：NILM42%(8/19)、 ASCUS27%(7/26)、ASCUS—H36%(4/11)、LSIL67%(3/9)、HSIL25%(1/4)、SCC0(0/3)。 NILM、ASCUS、ASCUS-H组相比,HPV L1壳蛋白的阳性表达率间的差异无统计学意义(P0.05)。而在LSIL、HSIL组与SCC组中比较,HPV L1壳蛋白的阳性表达率差异有统计学意义(均P0.05)。97名汉族患者中L1的表达阳性率为：NILM39%(15/38)、 ASCUS17%(6/35)、ASCUS—21%(4/19)、LSIL80%(1/5)、HSIL0(0/3)、SCC0(0/1)。 NILM、ASCUS、ASCUS-H组相比,HPV L1壳蛋白的阳性表达率间的差异无统计学意义(P0.05)。而在LSIL、HSIL组与SCC组中比较,HPV L1壳蛋白的阳性表达率差异有统计学意义(均P0.05)。两个民族在同级别病变之间L1蛋白的表达率比较差异无统计学意义(P0.05)。结论：HPV L1壳蛋白在HPV阳性的维、汉族患者宫颈病变中的阳性表达率均随着病变程度加重呈下降趋势(LSIL到HSIL),有望成为预测宫颈癌前病变进展的生物标志物。HPV L1壳蛋白在维、汉族同级别宫颈病变间的表达无差异。
[Abstract]:Objective: to investigate the expression, significance and difference of HPV L1 protein in cervical liquid-based cytological specimens of women from Uygur and Han nationalities in Xinjiang, and to provide a theoretical basis for the prevention and treatment of cervical lesions in women in Xinjiang. Methods: from December 2011 to October 2012, 182 female patients of Han nationality were selected from the people's Hospital of Xinjiang Uygur Autonomous region. Immunohistochemical SP method was used to detect the expression of Ll protein in HPV positive cervical liquid-based cytology samples. Results: the positive rate of L1 expression in 85 Uygur patients was NILM42% (8 / 19), ASCUS27% (7 / 26), ASCUS-H36% (4 / 11), LSIL67% (3 / 9), HSIL25% (1 / 4), SCC0 (0 / 3). There was no significant difference in positive expression rate of HPV L1 protein between NILM,ASCUS,ASCUS-H group and HPV group (P0.05). Compared with SCC group, the positive expression rate of HPV L1 shell protein in LSIL,HSIL group was significantly higher than that in SCC group (P0.05). The positive rates of L1 protein expression in 97 Han patients were NILM39% (15 / 38), ASCUS17% (6 / 35), NILM39% (15 / 38), ASCUS17% (6 / 35). ASCUS-21% (4 / 19), LSIL80% (1 / 5), HSIL0 (0 / 3), SCC0 (0 / 1). There was no significant difference in positive expression rate of HPV L1 protein between NILM,ASCUS,ASCUS-H group and HPV group (P0.05). The positive expression rate of HPV L1 protein in LSIL,HSIL group was significantly higher than that in SCC group (P0.05). There was no significant difference in the expression rate of L1 protein between the two nationalities in the same grade lesion (P0.05). Conclusion: the positive expression of HPV L1 protein in HPV and cervical lesions in Han nationality patients showed a decreasing trend (LSIL to HSIL),). It is expected to be a biomarker to predict the progression of cervical precancerous lesions. There is no difference in the expression of HPV L1 shell protein between the same grade cervical lesions in the Han nationality.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.33

【参考文献】