上皮性卵巢癌患者外周血及癌组织中KISS1 mRNA的表达及其临床意义

发布时间：2018-12-19 09:50

【摘要】：目的:探讨上皮性卵巢癌(epithelial ovarian cancer,EOC)患者外周血、癌组织中KISS1 mRNA的表达及其临床意义。方法:RT-PCR检测40例EOC、20例交界性卵巢肿瘤、20例良性卵巢肿瘤以及20例正常卵巢的组织及其术前静脉血中KISS1 mRNA的表达,分析KISS1 mRNA在4种组织间的表达差异性及在卵巢癌中的表达与临床病理特征的关系。结果:EOC组织及外周血KISS1 mRNA的表达量均高于良性肿瘤以及正常对照,差异有统计学意义(P0.001);EOC组织及外周血KISS1 mRNA的表达量与组织分化、临床分期、有无转移、术后残留病灶大小有关(P0.05);KISS1 mRNA在EOC原发病灶中的表达量高于其在对应转移灶中的表达,差异有统计学意义(P=0.001);外周血与卵巢组织中KISS1 mRNA的表达水平呈正相关关系(r=0.669,P0.001)。结论:KISS1基因可能参与了EOC的发生与发展,外周血KISS1 mRNA可作为卵巢癌分化程度、分期、复发及转移的生物学标志。
[Abstract]:Objective: to investigate the expression of KISS1 mRNA in peripheral blood and cancer tissues of patients with epithelial ovarian cancer (epithelial ovarian cancer,EOC) and its clinical significance. Methods: RT-PCR was used to detect the expression of KISS1 mRNA in 40 cases of borderline ovarian tumors, 20 cases of benign ovarian tumors and 20 cases of normal ovarian tissues. To analyze the expression difference of KISS1 mRNA in four kinds of tissues and the relationship between the expression of KISS1 mRNA and clinicopathological features. Results: the expression of KISS1 mRNA in EOC and peripheral blood was significantly higher than that in benign tumors and normal controls (P0. 001). The expression of KISS1 mRNA in EOC and peripheral blood was related to tissue differentiation, clinical stage, metastasis and the size of residual focus after operation (P0.05). The expression of KISS1 mRNA in the primary tumor of EOC was higher than that in the corresponding metastatic tumor (P0. 001), and the expression of KISS1 mRNA in peripheral blood was positively correlated with the expression of KISS1 mRNA in ovarian tissue (rr 0. 669 + P0. 001). Conclusion: KISS1 gene may be involved in the occurrence and development of EOC. Peripheral blood KISS1 mRNA can be used as a biological marker for differentiation, staging, recurrence and metastasis of ovarian cancer.
【作者单位】：南通大学第二附属医院妇产科;南通大学组织胚胎学教研室;
【基金】：南通市社会事业科技创新与示范计划项目资助(No.HS2013039)~~
【分类号】：R737.31

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