血管内皮特异性过表达microRNA-155对大鼠妊娠过程的影响

发布时间：2018-12-25 12:38

【摘要】：目的 :建立血管内皮特异过表达microRNA-155(miR-155)转基因大鼠模型并观察其血压、蛋白尿等表型及妊娠负荷施加后的变化。方法:把外源miR-155基因插入血管内皮细胞特异表达VE-cadherin启动子下游,构建转基因表达载体,将所得载体线性化后,通过受精卵显微注射技术,构建miR-155转基因大鼠,并利用特异引物PCR法鉴定转基因大鼠基因型。通过尾套法、生化检测、组织学检查对未孕和妊娠雌性转基因大鼠的表型进行初步分析。结果:血管内皮特异性过表达miR-155转基因大鼠在胸主动脉、腹主动脉、肾脏、心脏组织miR-155表达较野生型鼠增高2.3、3.3、2.9、3.3倍。未妊娠时miR-155转基因雌性大鼠血压、尿蛋白、血管壁和肾脏结构与非转基因型大鼠比较,差异均无统计学意义。但妊娠后miR-155转基因雌性大鼠血压轻度增高、蛋白尿增加、胎儿生长受限发生率增加,胸/腹主动脉的血管壁结构紊乱和肾小管上皮空泡样变等。结论:血管内皮特异性miR-155转基因大鼠妊娠负荷增加后,表现出子痫前期表型。
[Abstract]:Aim: to establish a vascular endothelial specifically expressed microRNA-155 (miR-155) transgenic rat model and observe the changes of blood pressure proteinuria phenotypes and pregnancy load. Methods: exogenous miR-155 gene was inserted into the downstream of vascular endothelial cell specifically expressing VE-cadherin promoter, and the transgenic expression vector was constructed. After linearization of the vector, miR-155 transgenic rats were constructed by microinjection of fertilized eggs. The genotypes of transgenic rats were identified by specific primer PCR method. The phenotypes of non-pregnant and pregnant female transgenic rats were preliminarily analyzed by tail sleeve method, biochemical examination and histological examination. Results: the expression of miR-155 in thoracic aorta, abdominal aorta, kidney and heart of miR-155 transgenic rats was higher than that of wild type rats. There was no significant difference in blood pressure, urine protein, vascular wall and renal structure between miR-155 transgenic female rats and non-transgenic rats. However, the blood pressure, proteinuria, fetal growth restriction, vascular wall structure of thoracic / abdominal aorta and tubular epithelial vacuolation were slightly increased after pregnancy in miR-155 transgenic female rats. Conclusion: vascular endothelial specific miR-155 transgenic rats showed preeclampsia phenotype after pregnancy load increased.
【作者单位】：南京医科大学附属鼓楼临床医学院妇产科;
【基金】：国家自然科学基金(81370724)
【分类号】：R714.1

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