Synaptopodin参与低分子肝素改善子痫前期大鼠蛋白尿的机制研究

发布时间：2019-01-04 20:35

【摘要】：目的:探讨synaptopodin在低分子肝素(LMWH)治疗子痫前期(PE)大鼠模型中的分子作用机制。方法:将PE孕鼠随机分为3组:(1)L-NAME组:于妊娠第9天起连续腹腔内注射亚硝基左旋精氨酸甲酯(L-NAME)至孕期结束;(2)L-LMWH组:注射LNAME的同时,孕15天起连续腹腔内注射LMWH 200IU/(kg·d)至孕期结束;(3)H-LMWH组:注射L-NAME的同时,孕15天起连续腹腔内注射LMWH 600IU/(kg·d)至孕期结束。动态监测其血压、24h尿蛋白总量及胎鼠体重变化。采用荧光定量PCR方法、免疫组化法和Western blot法检测肾脏组织中synaptopodin表达。结果:H-LMWH组中synaptopodin表达明显高于L-NAME组(P0.05),L-LMWH组和L-NAME组比较则无无明显变化(P0.05)。孕17和19天时,与L-NAME组比较,H-LMWH组和L-LMWH组孕鼠的蛋白尿和血压均明显降低,差异有统计学意义(P0.05);而胎鼠体重和胎鼠数无明显变化。结论:LMWH可能通过上调synaptopodin改善PE蛋白尿、降低血压。
[Abstract]:Aim: to investigate the molecular mechanism of synaptopodin in the treatment of preeclampsia (PE) rat model with low molecular weight heparin (LMWH) (LMWH). Methods: pregnant rats with PE were randomly divided into three groups: (1) L-NAME group: continuous intraperitoneal injection of nitroso L-arginine methyl ester (L-NAME) from the 9th day of pregnancy to the end of pregnancy; (2) L-LMWH group: at the same time of LNAME injection, LMWH 200IU/ (kg d) was continuously injected intraperitoneally from the 15th day of pregnancy to the end of pregnancy. (3) H-LMWH group: at the same time of L-NAME injection, LMWH 600IU/ (kg d) was continuously injected intraperitoneally from the 15th day of pregnancy to the end of pregnancy. The changes of blood pressure, 24 h urinary protein and fetal body weight were monitored dynamically. The expression of synaptopodin in renal tissues was detected by fluorescence quantitative PCR, immunohistochemistry and Western blot. Results: the expression of synaptopodin in H-LMWH group was significantly higher than that in L-NAME group (P0.05), but there was no significant difference between L-LMWH group and L-NAME group (P0.05). On the 17th and 19th day of pregnancy, the proteinuria and blood pressure of pregnant rats in H-LMWH group and L-LMWH group were significantly lower than those in L-NAME group (P0.05), but the body weight and the number of fetal mice had no significant change. Conclusion: LMWH may improve PE proteinuria and lower blood pressure by up-regulating synaptopodin.
【作者单位】：华中科技大学同济医学院附属同济医院妇产科;
【基金】：国家自然科学基金项目(No:81200451)
【分类号】：R714.244

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