基于气相色谱-质谱技术的代谢组学方法发现复发性流产标记物的研究

发布时间：2019-01-05 11:04

【摘要】：目的:基于气相色谱-质谱联用(GC-MS)技术研究复发性流产(RSA)的发病机制及可能的代谢标记物,为干预和治疗提供理论依据。方法:选择20例健康早孕孕妇(正常对照组)和20例RSA早孕孕妇(RSA组)为研究对象,用GC-MS方法对两组的尿液进行代谢轮廓分析,比较两组尿液代谢组分的差异。结果:两组的总离子流图显示:有一些谱峰信号在RSA组和正常对照组之间存在着明显的差异。采用PCA和PLS-DA对两组尿样代谢轮廓比较发现:两组的聚类基本完全分开,但大部分在三维空间的同一区域。采用PLS-DA方法建模:VIP值1的差异尿液代谢物共69个,匹配度80%的差异尿液代谢物共25个。结论:RSA孕妇和健康早孕孕妇的尿液代谢轮廓有明显的差异,这可能是RSA的发病机制所在;25个差异物质可能是RSA的代谢标记物。
[Abstract]:Objective: to study the pathogenesis and possible metabolic markers of recurrent abortion (RSA) by gas chromatography-mass spectrometry (GC-MS) in order to provide theoretical basis for intervention and treatment. Methods: 20 healthy early pregnant women (normal control group) and 20 RSA early pregnant women (RSA group) were selected as study objects. The metabolic profile of urine in the two groups was analyzed by GC-MS method, and the differences of urine metabolic components between the two groups were compared. Results: the total ion flow patterns of the two groups showed that there were significant differences between the RSA group and the normal control group. PCA and PLS-DA were used to compare the metabolic profiles of the two groups of urine samples. It was found that the clustering of the two groups was completely separate, but most of them were in the same area in three dimensional space. PLS-DA method was used to model: 69 different urine metabolites with VIP value 1 and 25 different urine metabolites with matching degree of 80%. Conclusion: there are significant differences in the profile of urine metabolism between RSA pregnant women and healthy early pregnant women, which may be the pathogenesis of RSA, and 25 differential substances may be the metabolic markers of RSA.
【作者单位】：湖南省妇幼保健院;
【分类号】：R714.21

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