RNA干扰CXCR4和CXCR7对子宫内膜癌HEC-1-A细胞生物学行为的影响

发布时间：2019-01-05 11:30

【摘要】：目的:探讨siRNA(small interference RNA)干扰趋化因子受体CXCR4和CXCR7对子宫内膜癌HEC-1-A细胞生物学行为的影响。方法:以LipofectamineTM2000脂质体为载体将siRNA转染至人子宫内膜癌HEC-1-A细胞株,以半定量RTPCR和Western blotting验证siRNA对CXCR4和CXCR7的干扰效果,以CCK-8法、流式细胞术和Transwell法分别检测CXCR4和CXCR7基因沉默对HEC-1-A细胞周期、增殖和侵袭能力的影响;结果:HEC-1-A细胞高表达CXCR4和CXCR7,合成的CXCR4-siRNA、CXCR7-siRNA、CXCR4/CXCR7-siRNA均可分别干扰HEC-1-A细胞内CXCR4、CXCR7、CXCR4/CXCR7基因或蛋白的表达;CXCR4和CXCR7基因的单独沉默或联合沉默均可导致HEC-1-A细胞增殖和侵袭能力的显著下降(P0.05),同时引发HEC-1-A细胞周期出现S期阻滞。结论:趋化因子受体CXCR4和CXCR7在HEC-1-A细胞的增殖和侵袭行为中发挥重要作用,CXCR4和CXCR7途径的抑制有望成为子宫内膜癌治疗的新靶点。
[Abstract]:Aim: to investigate the effects of siRNA (small interference RNA) interference chemokine receptors (CXCR4 and CXCR7) on the biological behavior of endometrial carcinoma HEC-1-A cells. Methods: siRNA was transfected into human endometrial carcinoma HEC-1-A cell line with LipofectamineTM2000 liposome as the carrier. The interference effect of siRNA on CXCR4 and CXCR7 was tested by semi-quantitative RTPCR and Western blotting. The effects of CXCR4 and CXCR7 gene silencing on the cell cycle, proliferation and invasion of HEC-1-A cells were detected by flow cytometry and Transwell. Results: the overexpression of CXCR4 in HEC-1-A cells and CXCR4-siRNA,CXCR7-siRNA,CXCR4/CXCR7-siRNA synthesis by CXCR7, could interfere with the expression of CXCR4,CXCR7,CXCR4/CXCR7 gene or protein in HEC-1-A cells, respectively. CXCR4 and CXCR7 gene silencing alone or combined silencing could significantly decrease the proliferation and invasion ability of HEC-1-A cells (P0.05) and induce S phase arrest of HEC-1-A cell cycle. Conclusion: chemokine receptors CXCR4 and CXCR7 play an important role in the proliferation and invasion of HEC-1-A cells. The inhibition of CXCR4 and CXCR7 pathway is expected to be a new target for the treatment of endometrial carcinoma.
【作者单位】：青岛大学附属医院妇科;
【基金】：山东省优秀中青年科学家科研奖励基金资助项目(No.BS2009SW002) 山东省自然科学基金资助项目(No.ZR2013HM012)~~
【分类号】：R737.33

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