宫颈脱落细胞HPVL1蛋白及HPVDNA载量对宫颈病变的预测价值

发布时间：2019-01-05 15:11

【摘要】：目的探讨宫颈脱落细胞HPVL1蛋白、人乳头瘤病毒(HPV)DNA载量对宫颈病变的预测作用。方法选取宫颈病变患者362例,宫颈刷采集宫颈脱落细胞,采用免疫化学方法检测HPVL1蛋白,HC2技术检测HPV DNA载量,根据宫颈组织病理学结果将患者分为慢性宫颈炎(190例)、宫颈上皮内瘤变Ⅰ期(CINⅠ,45例)、宫颈上皮内瘤变Ⅱ期(CINⅡ,43例)、宫颈上皮内瘤变Ⅲ期(CINⅢ,46例)和宫颈癌(38例);根据宫颈细胞学检查结果将脱落细胞分为未见异常细胞(NILM,122例),未明确性非典型鳞状上皮细胞(ASCUS,75例),可疑非典型鳞状上皮病变细胞(ASCH,52例),低度鳞状上皮内病变(LSIL,61例)和高度鳞状上皮内病变(HSIL,52例),比较不同宫颈病变HPVL1蛋白、HPV DNA载量的差异。结果 130例宫颈脱落细胞HPVL1蛋白阳性,阳性率为35.91%(130/362),其中HPVL1蛋白阳性率由大至小分别为:慢性宫颈炎CINⅠ期CINⅡ期CINⅢ期宫颈癌,NILMASCUSASCHLSILHSIL,不同宫颈病变组织病理学或细胞学类型比较差异具有统计学意义(P0.05),272例HPV DNA阳性,阳性率为75.14%,低度HPV DNA载量以慢性宫颈炎或NILM最多,高度HPV DNA载量以宫颈癌或HSIL最多,明显高于其他宫颈组织病理学或细胞学类型(P0.05)。结论 HPVL1蛋白在宫颈脱落细胞的表达随着宫颈病变程度加重而下调,HPV DNA载量随宫颈病变程度加重而上调,二者检测在宫颈病变程度的预测中具有重要的价值。
[Abstract]:Objective to investigate the predictive effect of HPVL1 protein and (HPV) DNA load of human papillomavirus (HPV) on cervical lesions. Methods Cervical exfoliated cells were collected from 362 patients with cervical lesions, HPVL1 protein was detected by immunocytochemical method and HPV DNA load was detected by HC2 technique. The patients were divided into chronic cervicitis (190 cases) according to the results of cervical histopathology. Cervical intraepithelial neoplasia stage 鈪，

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