HCMV感染对宫颈癌细胞Siha发生上皮-间质转化的作用

发布时间：2019-01-07 15:12

【摘要】：目的研究人巨细胞病毒(HCMV)感染对宫颈癌细胞Siha发生上皮-间质转化的作用及意义。方法实验组用HCMV AD169(MOI=5)和5ng/ml TGF-β1作用宫颈癌Siha细胞,同时将仅TGF-β1作用的Siha细胞作为对照组,分别在0、24、48、72h观察细胞形态的变化,应用RT-PCR检测E-钙粘蛋白和波形蛋白的转录水平,采用Western blot检测E-钙粘蛋白、波形蛋白和MMP-2的表达水平。结果实验组细胞于48h发生细胞形态学的变化,早于对照组的72h;实验组上皮细胞标记物E-钙粘蛋白的转录和表达水平较对照组显著下调(P0.01),间质细胞标记物波形蛋白的转录和表达较对照组显著上调(P0.01)且表达提前,MMP-2的表达较对照组显著上调(P0.01)且表达提前。结论 HCMV感染可促进宫颈癌细胞Siha发生化上皮-间质转化。
[Abstract]:Objective to study the effect and significance of human cytomegalovirus (HCMV) infection on epithelial-interstitial transformation of cervical cancer cell Siha. Methods HCMV AD169 (MOI=5) and 5ng/ml TGF- 尾 1 were used to treat cervical cancer Siha cells in the experimental group and Siha cells treated with TGF- 尾 1 alone were used as control group. The morphological changes of the cells were observed at 48 h after treatment. The transcription levels of E-cadherin and vimentin were detected by RT-PCR, and the expression of Ecadherin, vimentin and MMP-2 by Western blot. Results the morphological changes of the cells in the experimental group were observed at 48h, earlier than those in the control group (72 h). The transcription and expression level of E-cadherin in the experimental group was significantly lower than that in the control group (P0.01), and the expression of vimentin in the interstitial cells was up-regulated (P0.01), and the expression of vimentin in the interstitial cells was earlier than that in the control group (P0.01). The expression of MMP-2 was significantly up-regulated than that of control group (P0.01). Conclusion HCMV infection can promote the epithelial-interstitial transformation of Siha in cervical cancer cells.
【作者单位】：青岛大学医学院护理学院;青岛大学医学院病原生物学教研室;
【基金】：国家自然科学基金项目(No.81070501)
【分类号】：R737.33

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