人上皮性卵巢癌细胞系SKOV3-luc裸鼠皮下及原位移植瘤模型的建立与研究

发布时间：2019-01-18 16:00

【摘要】：目的:建立整体可视化人卵巢癌皮下和原位移植瘤模型,用于实时观察和分析人卵巢癌移植瘤发展和转移的规律,并比较两者的成瘤情况及生物学特性。方法:使用带有荧光素酶报告基因(Luciferase)的人上皮性卵巢癌细胞系SKOV3-luc分别接种于裸鼠右侧腋窝皮下和左侧卵巢内,建立人上皮性卵巢癌裸鼠皮下和原位移植瘤模型。实时观察卵巢癌细胞SKOV3-luc在裸鼠皮下和原位的生长情况,并切取肿瘤组织,应用病理组织学方法对裸鼠皮下移植瘤和原位移植瘤进行检测和评价。结果:人卵巢癌细胞系SKOV3-luc裸鼠皮下和原位移植瘤模型建立的成功率均较高。裸鼠皮下和左侧卵巢接种肿瘤细胞后,可通过活体成像实时观察肿瘤的生长情况,并且行定量分析肿瘤形成时间等。皮下移植瘤(皮下组)成瘤率为100%,左侧卵巢原位移植瘤(原位组)成功率为100%。注射后第7天,皮下组的肿瘤总荧光强度比值为(3.38±2.50)(Fold),原位组为(1.43±1.32)(Fold);第14天,皮下组为(15.73±11.70)(Fold),原位组为(6.44±7.26)(Fold),皮下组第7天和第14天的肿瘤总荧光强度比值均大于原位组(P0.05)。结论:人上皮性卵巢癌裸鼠皮下移植瘤和卵巢原位移植瘤建模成功率高,并能很好地反应肿瘤的生物学行为,实时荧光成像便于观察肿瘤体内生长情况,为研究卵巢癌发展和转移提供了可靠的观察模型。
[Abstract]:Objective: to establish a visualized subcutaneous and orthotopic transplanted tumor model of human ovarian cancer to observe and analyze the development and metastasis of human ovarian cancer transplantation tumor in real time, and to compare their tumorigenesis and biological characteristics. Methods: the human epithelial ovarian cancer cell line SKOV3-luc with luciferase reporter gene (Luciferase) was inoculated into the subcutaneous of right axilla and the left ovary of nude mice respectively to establish subcutaneous and orthotopic transplantation tumor models of human epithelial ovarian cancer in nude mice. The growth of ovarian cancer cell SKOV3-luc in subcutaneous and in situ in nude mice was observed in real time. The tumor tissue was removed and the subcutaneous transplanted tumor and orthotopic transplanted tumor were detected and evaluated by histopathological method. Results: the success rate of subcutaneous and orthotopic transplantation of human ovarian cancer cell line SKOV3-luc in nude mice was higher. After inoculating tumor cells subcutaneously and left ovary in nude mice, tumor growth could be observed in real time by in vivo imaging, and tumor formation time could be quantitatively analyzed. The rate of tumor formation was 100% in subcutaneous group and 100% in left ovarian orthotopic transplantation group. On the 7th day after injection, the ratio of total fluorescence intensity in subcutaneous group was (3.38 卤2.50) (Fold),) and (1.43 卤1.32) (Fold);) in in-situ group. On the 14th day, the ratio of total fluorescence intensity in the subcutaneous group (15.73 卤11.70) (Fold),) was (6.44 卤7.26) (Fold),) on the 7th day and the 14th day in the subcutaneous group was higher than that in the in situ group (P0.05). Conclusion: the models of subcutaneous transplanted tumor and orthotopic transplantation tumor of human epithelial ovarian cancer in nude mice have high success rate and can well reflect the biological behavior of tumor. Real-time fluorescence imaging is convenient to observe the growth of tumor in vivo. It provides a reliable observation model for the development and metastasis of ovarian cancer.
【作者单位】：同济大学附属第一妇婴保健院妇科;
【分类号】：R737.31

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