胎盘性激素结合球蛋白基因启动子n重复多态与妊娠期糖尿病关系研究

发布时间：2019-01-25 20:43

【摘要】：目的探讨胎盘性激素结合球蛋白(sex hormone-binding globulin,SHBG)基因启动子(TAAAA)n重复多态与妊娠期糖尿病(gestational diabetes mellitus,GDM)的关系。方法选择2011年1月至2013年2月在中国医科大学附属盛京医院单胎分娩女婴GDM孕妇129例(GDM组)和健康孕妇119例(对照组),采用基因片段分析加测序法检测胎盘SHBG基因启动子(TAAAA)n重复多态长度和重复次数,两组间进行比较。结果两组孕妇胎盘均检测到(TAAAA)n重复次数为6、7、8、9、10次重复5个等位基因,6/6、6/7、6/8、6/9、6/10、7/7、7/8、7/9、7/10、8/8、8/9、8/10、9/9、9/10共14种基因型。两组间5种等位基因构成比及各等位基因频率差异均无统计学意义(P0.05)。GDM组胎盘长链重复基因型频率虽然高于短链,但差异无统计学意义(χ~2=2.149,P=0.143)。结论胎盘SHBG基因启动子(TAAAA)n重复多态与妊娠期糖尿病无关。
[Abstract]:Objective to investigate the relationship between (TAAAA) n repeat polymorphism of placental sex hormone binding globulin (sex hormone-binding globulin,SHBG) gene promoter and gestational diabetes mellitus (gestational diabetes mellitus,GDM). Methods from January 2011 to February 2013, 129 GDM pregnant women (GDM group) and 119 healthy pregnant women (control group) in Shengjing Hospital affiliated to China Medical University were selected. The polymorphic length and number of (TAAAA) n repeats of placental SHBG gene promoter were detected by gene fragment analysis and sequencing, and the comparison was made between the two groups. Results in the placenta of both groups, the number of (TAAAA) n repeats was 6, 7, 8, 9, 10, 10, 5 alleles, 6 / 6, 6, 6, 7, 6, 6, 6, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, There was no significant difference in the composition ratio of five alleles and the frequency of each allele between the two groups (P0.05). The frequency of long chain repeat genotype of placenta in). GDM group was higher than that in short chain group, but there was no significant difference between them (蠂 ~ 2. 149%, P < 0. 143). Conclusion the (TAAAA) n repeat polymorphism of placental SHBG gene promoter is not associated with gestational diabetes mellitus.
【作者单位】：中国医科大学附属盛京医院妇产科;
【基金】：国家自然科学基金(81170591;81300511)
【分类号】：R714.256

【相似文献】