吉林松原地区宫颈癌人乳头瘤病毒基因检测分析

发布时间：2019-02-11 08:34

【摘要】：目的探讨吉林松原地区宫颈癌患者的人乳头瘤病毒(HPV)感染情况及与宫颈癌临床生物行为之间的关系。方法采用凯普医用核酸分子杂交技术及其配套试剂对2010年2月至2015年5月吉林油田总医院收治的142例宫颈癌患者行HPV分型检测,并统计分析。结果 138例HPV阳性(97.18%),其中单一感染117例,多重感染21例,二者阳性率差异有统计学意义(P0.01)。共检测出15种基因型,单一感染10种,阳性率由高至低依次为HPV16(66.67%)、HPV18(5.80%)、HPV31(2.90%)、HPV45(1.45%)、HPV33(2.17%)、HPV58(1.45%)、HPV59(1.45%)、HPV6(1.45%)、HPV56(0.72%)和CP8304(0.72%)。而HPV11(2.90%)、HPV39(2.17%)、HPV52(1.45%)、HPV66(0.72%)、HPV68(0.72%)出现在多重感染中。HPV16总阳性率为77.54%,明显高于其他型别(P0.01)。HPV18总阳性率(8.70%)与HPV31、HPV33、HPV45比较差异无统计学意义(P0.05)。HPV阳性率在宫颈癌各临床分期、组织学分级间差异无统计学意义(P0.05)。结论吉林松原地区宫颈癌患者主要是HPV单一感染,以HPV16为主,其次是HPV18、HPV31、HPV45;未发现多重感染增加宫颈癌的发生风险;HPV感染不影响宫颈癌的进展及肿瘤细胞的分化。
[Abstract]:Objective to investigate the relationship between human papillomavirus (HPV) infection and clinical biological behavior of cervical cancer in Songyuan, Jilin province. Methods HPV typing was performed in 142 patients with cervical cancer treated in General Hospital of Jilin Oilfield from February 2010 to May 2015 by using Kepper Medical Nucleic Acid Molecular Hybridization technique and its matching reagents. Results the positive rate of HPV in 138 cases (97.18%) was significantly higher than that in single infection (117 cases) and multiple infection (21 cases) (P0.01). A total of 15 genotypes were detected, 10 of which were single infection. The positive rates from high to low were HPV16 (66.67%), HPV18 (5.80%), HPV31 (2.90%), HPV45 (1.45%), HPV33 (2.17%), HPV58 (1.45%). HPV59 (1.45%), HPV6 (1.45%), HPV56 (0.72%) and CP8304 (0.72%). HPV11 (2.90%), HPV39 (2.17%), HPV52 (1.45%), HPV66 (0.72%) and HPV68 (0.72%) were found in multiple infections. The total positive rate of HPV16 was 77.54%. The total positive rate of HPV18 (8.70%) was not significantly different from that of HPV31,HPV33,HPV45 (P0.05) in all clinical stages of cervical cancer. There was no significant difference in histological grade (P0.05). Conclusion Cervical cancer patients in Songyuan area of Jilin Province are mainly HPV single infection, mainly HPV16, followed by HPV18,HPV31,HPV45; did not find multiple infection to increase the risk of cervical cancer, HPV infection does not affect the progression of cervical cancer and differentiation of tumor cells.
【作者单位】：吉林油田总医院妇科;
【分类号】：R737.33

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