妊娠期糖尿病大鼠胰腺组织中p-PERK、ATF4和CHOP蛋白的表达
发布时间：2019-02-15 04:23
【摘要】：目的:探讨磷酸化蛋白激酶R样内质网激酶(p-PERK)、C/EBP同源蛋白(CHOP)、ATF4蛋白的表达与高脂高糖诱导的妊娠期糖尿病大鼠胰岛β细胞凋亡的关系。方法:30只雌性SD大鼠分为模型组(高脂高糖喂养)和对照组(普食喂养),每组15只,妊娠第21天行静脉糖耐量及静脉胰岛素释放实验;取胰腺组织行HE染色和病理组织学观察,TUNEL法检测胰岛β细胞凋亡情况,免疫组化法检测胰腺组织中p-PERK蛋白的表达,Western blot检测胰腺组织中ATF4及CHOP蛋白的表达。结果:模型组大鼠第一时相胰岛素分泌受抑制,胰岛素分泌峰值延迟;病理组织学可见模型组大鼠胰岛形态欠规则,数目减少不明显,部分细胞空泡变性,毛细血管扩张、充血,有出血发生。模型组大鼠胰腺组织细胞凋亡率较对照组升高(t=21.164,P0.001)。与对照相比,模型组大鼠胰腺组织p-PERK、ATF4及CHOP蛋白表达水平明显升高(t=44.178、49.249、39.664,P0.001);模型组大鼠胰腺组织中ATF4与CHOP蛋白的表达呈正相关(r=0.810,P=0.004)。结论:PERK、ATF4可能参与妊娠期糖尿病大鼠胰岛β细胞凋亡的发生。
[Abstract]:Aim: to investigate the relationship between the expression of phosphorylated protein kinase R-like endoplasmic reticulum kinase (p-PERK), C/EBP homologous protein (CHOP), ATF4 protein and islet 尾 -cell apoptosis in gestational diabetic rats induced by hyperlipidemia and hyperglycemia. Methods: thirty female SD rats were divided into two groups: model group (fed with high fat and high sugar) and control group (15 rats in each group). HE staining and histopathological observation were performed in pancreatic tissues. Apoptosis of islet 尾 cells was detected by TUNEL method. The expression of p-PERK protein in pancreatic tissue was detected by immunohistochemical method. The expression of ATF4 and CHOP protein in pancreatic tissue was detected by, Western blot. Results: the first phase of insulin secretion was inhibited and the peak value of insulin secretion was delayed in the model group. Histopathology showed that the number of islets in the model group was not regular, the number of islets was not obvious, some cells were vacuolar degeneration, capillary dilatation, hyperemia and haemorrhage occurred. The apoptosis rate of pancreatic tissue in the model group was higher than that in the control group (t = 21.164, P 0.001). Compared with the control group, the expression of p-PERKN ATF4 and CHOP protein in the model group was significantly higher than that in the control group. There was a positive correlation between the expression of ATF4 and CHOP protein in the pancreatic tissue of the model group (r = 0.810 P0. 004). Conclusion: PERK,ATF4 may be involved in the apoptosis of islet 尾 cells in gestational diabetic rats.
【作者单位】： 郑州大学第二附属医院内分泌科;郑州大学第二附属医院神经内科;
【基金】：河南省医学科技攻关计划项目201303083
【分类号】：R714.256
本文编号：2422959
[Abstract]:Aim: to investigate the relationship between the expression of phosphorylated protein kinase R-like endoplasmic reticulum kinase (p-PERK), C/EBP homologous protein (CHOP), ATF4 protein and islet 尾 -cell apoptosis in gestational diabetic rats induced by hyperlipidemia and hyperglycemia. Methods: thirty female SD rats were divided into two groups: model group (fed with high fat and high sugar) and control group (15 rats in each group). HE staining and histopathological observation were performed in pancreatic tissues. Apoptosis of islet 尾 cells was detected by TUNEL method. The expression of p-PERK protein in pancreatic tissue was detected by immunohistochemical method. The expression of ATF4 and CHOP protein in pancreatic tissue was detected by, Western blot. Results: the first phase of insulin secretion was inhibited and the peak value of insulin secretion was delayed in the model group. Histopathology showed that the number of islets in the model group was not regular, the number of islets was not obvious, some cells were vacuolar degeneration, capillary dilatation, hyperemia and haemorrhage occurred. The apoptosis rate of pancreatic tissue in the model group was higher than that in the control group (t = 21.164, P 0.001). Compared with the control group, the expression of p-PERKN ATF4 and CHOP protein in the model group was significantly higher than that in the control group. There was a positive correlation between the expression of ATF4 and CHOP protein in the pancreatic tissue of the model group (r = 0.810 P0. 004). Conclusion: PERK,ATF4 may be involved in the apoptosis of islet 尾 cells in gestational diabetic rats.
【作者单位】： 郑州大学第二附属医院内分泌科;郑州大学第二附属医院神经内科;
【基金】：河南省医学科技攻关计划项目201303083
【分类号】：R714.256
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