重度子痫前期胎盘中CD142的表达与其启动子区甲基化程度的关系

发布时间：2019-02-18 08:02

【摘要】：目的探究CD142在重度子痫前期孕妇胎盘中的表达情况及其与启动子区域甲基化之间的关系。方法本研究采用实时荧光定量PCR、免疫组化、蛋白质印迹检测24例重度子痫前期孕妇和24例正常妊娠孕妇胎盘中CD142基因的表达情况;采用甲基化特异性PCR检测CD142启动子区域的甲基化情况。结果病例组胎盘中CD142mRNA相对含量(1.45±0.42)高于正常妊娠孕妇(0.25±0.28),差异有统计学意义(P0.05);病例组胎盘中CD142蛋白表达量(0.857±0.043)高于正常晚孕者(0.248±0.035),差异有统计学意义(P0.05);病例组胎盘组织中CD142启动子区域的甲基化阳性率低于对照组(29.2%vs.100.0%,χ2=36.11,P0.001),非甲基化阳性率高于正常组(100.0%vs.20.8%,χ2=29.85,P0.001);CD142的蛋白表达水平与其启动子区域的甲基化程度呈负相关(r=-0.909,P0.05)。结论 CD142通过甲基化调控蛋白的表达在重度子痫前期的发病过程中发挥重要作用。
[Abstract]:Objective to investigate the expression of CD142 in placenta of severe preeclampsia and its relationship with promoter region methylation. Methods the expression of CD142 gene in placenta of 24 severe preeclampsia pregnant women and 24 normal pregnant women was detected by real-time fluorescence quantitative PCR, immunohistochemistry and Western blot. Methylation of CD142 promoter region was detected by methylation specific PCR. Results the relative content of CD142mRNA in placenta of the case group (1.45 卤0.42) was higher than that of the normal pregnant women (0.25 卤0.28), the difference was statistically significant (P0.05). The expression of CD142 protein in placenta of case group (0.857 卤0.043) was higher than that of normal late pregnancy (0.248 卤0.035), the difference was statistically significant (P0.05). The positive rate of methylation in the placental tissue of the case group was lower than that in the control group (29.2vs.100.0g, 蠂 ~ 2 + 36.11p 0.001), and the positive rate of non-methylation was higher than that of the normal group (100.0vs.20.8cm, 29.85). (P0.001); The protein expression level of CD142 was negatively correlated with the methylation degree of its promoter region (r-0.909 P 0.05). Conclusion CD142 plays an important role in the pathogenesis of severe preeclampsia through the expression of methylated regulatory protein.
【作者单位】：西安交通大学第一附属医院妇产科;西安交通大学第一附属医院周围血管科;西安交通大学第一附属医院乳腺外科;山阳县医院妇产科;
【基金】：中央高校基本科研业务费资助(No.1191320110) 西安交通大学第一附属医院基金项目(No.YK201528) 西安交通大学临床新技术项目(No.XJLS-2015-287)~~
【分类号】：R714.244

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