SIRT2在浆液性卵巢癌细胞系中的表达及其对增殖、迁移和侵袭的影响

发布时间：2019-02-25 09:07

【摘要】：目的:研究SIRT2在卵巢表层上皮(ovarian surface epithelium,OSE)及浆液性卵巢癌(serous ovarian carcinoma,SOC)细胞系中的表达情况并从细胞增殖、迁移和侵袭这3个方面探讨SIRT2对SOC恶性生物学行为的影响。方法:运用Western blot技术检测OSE和SOC细胞系中SIRT2蛋白的表达水平;设计靶向SIRT2的siRNA,构建SIRT2过表达载体,分别瞬时转染OSE细胞系HOSEpi C和SOC细胞系HO8910,平板克隆形成实验和CCK-8实验研究SIRT2对细胞生长的影响;细胞划痕实验考察SIRT2在SOC细胞迁移中的作用;Transwell实验研究SIRT2对SOC细胞侵袭能力的影响。结果:5株SOC细胞系中SIRT2的表达水平显著低于OSE细胞系。在HOSEpi C细胞中沉默SIRT2,细胞克隆形成数增多,细胞活力增强。相反,在HO8910细胞中过表达SIRT2,细胞克隆形成数减少,细胞活力降低。沉默SIRT2促进HOSEpi C细胞的迁移,而过表达SIRT2则抑制HO8910细胞的迁移。沉默SIRT2的HOSEpi C细胞侵袭能力明显增加,而过表达SIRT2的HO8910细胞侵袭能力则显著降低。结论:SIRT2在SOC细胞中表达显著下调。SIRT2在OSE细胞中是肿瘤抑制蛋白,抑制细胞增殖、迁移和侵袭。
[Abstract]:Objective: to investigate the expression of SIRT2 in ovarian surface epithelial (ovarian surface epithelium,OSE and serous ovarian cancer (serous ovarian carcinoma,SOC cell lines and to explore the effects of SIRT2 on the malignant biological behavior of SOC from the aspects of cell proliferation, migration and invasion. Methods: Western blot technique was used to detect the expression of SIRT2 protein in OSE and SOC cell lines. The overexpression vector of SIRT2 was constructed by designing siRNA, targeting SIRT2, and the effect of SIRT2 on cell growth was studied by transient transfection of OSE cell line HOSEpi C and SOC cell line HO8910,. The effect of SIRT2 on the migration of SOC cells and the effect of SIRT2 on the invasiveness of SOC cells were investigated by cell scratch assay and Transwell assay. Results: the expression of SIRT2 in 5 SOC cell lines was significantly lower than that in OSE cell lines. Silencing SIRT2, cells in HOSEpi C cells increased the number of clones and increased cell viability. On the contrary, the number of clones and cell viability of overexpressed SIRT2, cells decreased in HO8910 cells. Silencing SIRT2 promoted the migration of HOSEpi C cells, while overexpression of SIRT2 inhibited the migration of HO8910 cells. The invasiveness of HOSEpi C cells silenced by SIRT2 was significantly increased, while that of HO8910 cells overexpression of SIRT2 was significantly decreased. Conclusion: the expression of SIRT2 is down-regulated in SOC cells, and SIRT2 is a tumor suppressor protein in OSE cells, which inhibits cell proliferation, migration and invasion.
【作者单位】：复旦大学附属妇产科医院;上海市女性生殖内分泌相关疾病重点实验室;
【基金】：上海市科学技术委员会科研计划(No.14ZR1404100)
【分类号】：R737.31

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