硒化合物对宫颈癌HeLa细胞增殖、迁移及粘附功能影响的研究

发布时间：2019-03-03 16:10

【摘要】：目的探讨甲基硒酸(Me Se A)、硒代蛋氨酸(Se Met)和甲基硒代半胱氨酸(Me Se Cys)对宫颈癌He La细胞的增殖、迁移及粘附功能的影响。方法体外培养He La细胞,3μmol/L的Me Se A、Se Met和Me Se Cys处理He La细胞12~72 h,分别采用MTT法、划痕实验和体外基质粘附实验检测He La细胞的增殖、迁移及粘附功能。结果与对照细胞相比,Me Se A处理组He La细胞的增殖速度明显降低(P0.01)。Me Se A处理组He La细胞在4 h和8 h的迁移抑制率分别为34%(P0.05)和26%(P0.05);Se Met处理组He La细胞在4 h和8 h的迁移抑制率分别为18%和13%;Me Se Cys处理组He La细胞在4 h和8 h的迁移抑制率分别为28%(P0.05)和5%。Me Se A、Se Met和Me Se Cys对He La细胞粘附功能的抑制率分别为36%(P0.01)、25%和49%(P0.01)。结论 Me Se A可以更好地抑制He La细胞的增殖和迁移,而Me Se Cys对He La细胞的粘附功能的抑制作用更强。
[Abstract]:Aim to investigate the effects of (Me Se A), selenomethionine (Se Met) and (Me Se Cys) on proliferation, migration and adhesion of cervical cancer He La cells. Methods He La cells were cultured in vitro and treated with 3 渭 mol / L Me Se A, se Met and Me Se Cys for 12 h. The proliferation, migration and adhesion function of He La cells were detected by MTT assay, scratch test and matrix adhesion assay in vitro, respectively. Results the proliferation rate of He La cells in, Me Se A treated group was significantly lower than that in control group (P0.01). The migration inhibition rates of He La cells in). Me Se A treated group at 4 h and 8 h were 34% (P0.05) and 26% (P0.05), respectively. The migration inhibition rates of He La cells treated with Se Met at 4 h and 8 h were 18% and 13%, respectively. The migration inhibition rates of He La cells at 4 h and 8 h in Me Se Cys group were 28% (P0.05) and 5%.Me Se A, respectively. The inhibitory rates of se Met and Me Se Cys on He La cell adhesion were 36% (P0.01). 25% and 49% (P0.01). Conclusion Me Se A can inhibit the proliferation and migration of He La cells better than Me Se Cys can inhibit the adhesion of He La cells.
【作者单位】：中国疾病预防控制中心营养与健康所;山东商业职业技术学院食品药品学院;
【基金】：国家自然科学基金(No.81372989) 北京市教委科技发展面上项目(No.SQKM201210025002) 中国营养学会伊利营养与健康研究基金(No.2013-013)
【分类号】：R737.33

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