力达霉素对人宫颈癌Caski细胞增殖的影响及其机制

发布时间：2019-03-13 08:09

【摘要】：目的探讨力达霉素对人宫颈癌Caski细胞增殖的影响及其可能的机制。方法将对数生长期的人宫颈癌Caski细胞随机分为低、中、高浓度组及对照组,低、中、高浓度组均加入含力达霉素的RPMI 1640完全培养基100μL,分别调整其终浓度为1、2.5、5 ng/m L,对照组仅加入等体积RPMI 1640完全培养基。作用24 h,采用MTT法检测细胞增殖能力(以吸光度值表示),计算细胞生存率;采用流式细胞术检测细胞周期及线粒体膜电位降低比例;透射电子显微镜下观察细胞形态学改变,计算细胞凋亡率及裂亡率。结果低、中、高浓度组细胞增殖能力及细胞生存率均低于对照组,且高浓度组降低更明显(P均0.01)。对照组及中浓度组S期细胞比例均高于低、高浓度组(P均0.05)。低、中、高浓度组G0/G1期细胞比例均低于对照组,G2/M期细胞比例均高于对照组,且中、高浓度组变化更明显(P均0.05)。低、中、高浓度组及对照组细胞线粒体膜电位降低比例分别为(34.77±5.33)%、(37.87±5.73)%、(18.37±3.91)%、(4.17±0.65)%;低、中、高浓度组细胞线粒体膜电位降低比例均高于对照组,且低、中浓度组均高于高浓度组(P均0.05)。透射电镜下可见对照组细胞形态正常,低、中、高浓度组存在多种形态的死亡细胞,包括凋亡、坏死、裂亡的细胞。对照组及高、低、中浓度组细胞凋亡率及裂亡率均依次升高,两组间比较P均0.05。结论力达霉素可抑制人宫颈癌Caski细胞增殖,并呈浓度依赖性,其机制可能与诱导细胞凋亡或裂亡有关。
[Abstract]:Objective to investigate the effect of lidamycin on the proliferation of human cervical cancer Caski cells and its possible mechanism. Methods Human cervical cancer Caski cells in logarithmic growth phase were randomly divided into low, middle, high concentration and control groups, low, middle and high concentration groups were added with RPMI 1640 complete medium containing lidamycin 100 渭 L, and their final concentrations were adjusted to 1,2.5 and 5 ng/m / L, respectively. In the control group, only equal volume RPMI 1640 complete medium was added to the control group. After 24 h, cell proliferation ability (expressed as absorbance value) was measured by MTT method, cell survival rate was calculated by flow cytometry, cell cycle and mitochondrial membrane potential reduction ratio were measured by flow cytometry. The morphological changes of the cells were observed under the transmission electron microscope, and the apoptosis rate and the cleavage rate of the cells were calculated. Results the cell proliferation ability and cell survival rate in the high concentration group were lower than those in the control group, and decreased more significantly in the high concentration group than in the control group (P 0.01). The proportion of S phase cells in the control group and the medium concentration group was higher than that in the low concentration group, and in the high concentration group (P 0.05). In low, middle and high concentration groups, the percentage of G0/G1 phase cells was lower than that of control group, and the proportion of G _ 2 / M phase cells in high concentration group was higher than that of control group, and the change was more obvious in middle and high concentration groups (all P0.05). The percentage of mitochondrial membrane potential decreased by (34.77 卤5.33)%, (37.87 卤5.73)%, (18.37 卤3.91)%, (4.17 卤0.65)% in high concentration group and control group, respectively. The percentage of mitochondrial membrane potential decreased in the high concentration group was higher than that in the control group, and was lower in the medium concentration group than in the high concentration group (all P 0.05). Under the transmission electron microscope, there were many kinds of dead cells, including apoptosis, necrosis and dehiscence, in the normal, low, middle and high concentration groups of the control group. In control group and high, low, middle concentration groups, the apoptosis rate and cleavage rate were increased in turn, and the difference between the two groups was 0. 05% (P < 0. 05). Conclusion lidamycin can inhibit the proliferation of human cervical cancer Caski cells in a concentration-dependent manner, and its mechanism may be related to the induction of apoptosis or apoptosis.
【作者单位】：三峡大学肿瘤微环境与免疫治疗湖北省重点实验室;
【基金】：国家自然科学基金资助项目(81372265)
【分类号】：R737.33

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