妊娠期肝内胆汁淤积症孕妇胎盘组织HDAC3的表达及意义研究

发布时间：2019-03-28 20:35

【摘要】：目的:探讨组蛋白去乙酰化酶3(histone deacetylase 3,HDAC3)在妊娠期肝内胆汁淤积症(intrahepatic cholestasis of pregnancy,ICP)孕妇胎盘组织中的表达及意义。方法:2012年3月至2013年5月在重庆医科大学附属第一医院选择ICP孕妇41例为研究对象,包括ICP轻度组(19例)、ICP重度组(22例),以同期行择期剖宫产术的20例正常孕妇为对照组。免疫组化法检测胎盘组织中HDAC3的定位及表达,蛋白印迹(Western blot)技术检测胎盘组织中HDAC3蛋白的表达水平,实时荧光定量PCR(real-time PCR)技术检测胎盘组织中HDAC3 m RNA的表达水平。结果:(1)ICP重度组孕妇血清中肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、总胆汁酸(total bile acid,TBA)及谷草转氨酶(glutamic-oxaloacetic transaminase,AST)、谷丙转氨酶(glutamic-pyruvic transaminase,ALT)水平明显高于轻度组和正常对照组,差异有统计学意义(P0.05)。(2)ICP重度组和轻度组胎盘组织中HDAC3蛋白和m RNA的表达水平均低于对照组,分别比较差异均有统计学意义(P0.05)。(3)胎盘组织中HDAC3蛋白表达水平与血清中TNF-α、TBA及ALT、AST水平呈负相关。结论:HDAC3表达下调可能与ICP发病及肝功能损害有关。
[Abstract]:Aim: to investigate the expression and significance of histone deacetylase 3 (HDAC 3) in placenta of pregnant women with intrahepatic cholestasis of pregnancy (intrahepatic cholestasis of pregnancy,ICP). Methods: from March 2012 to May 2013, 41 pregnant women with ICP were selected from the first affiliated Hospital of Chongqing Medical University, including ICP mild group (19 cases of severe), ICP group (22 cases). Twenty normal pregnant women who underwent elective cesarean section at the same time were used as control group. Immunohistochemical method was used to detect the location and expression of HDAC3, Western blot (Western blot) was used to detect the expression level of HDAC3 protein in placenta, and real-time fluorescence quantitative PCR (real-time PCR) technique was used to detect the expression of HDAC3 m RNA in placental tissue. Results: (1) tumor necrosis factor 伪 (tumor necrosis factor- 伪 (TNF- 伪), total bile acid (total bile acid,TBA, glutamic oxaloacetic transaminase (glutamic-oxaloacetic transaminase,AST) and glutamic pyruvic transaminase (glutamic-pyruvic transaminase,) in serum of pregnant women with severe ICP were measured. The expression of HDAC3 protein and m RNA in placental tissue of severe ICP group and mild ICP group were significantly lower than those of control group (P0.05). (2), and the level of ALT was significantly higher than that of mild group and mild group (P0.05). (2). The expression of HDAC3 protein in placental tissue was negatively correlated with serum TNF- 伪, TBA and ALT,AST levels (P0.05). (3). Conclusion: the down-regulation of HDAC3 expression may be related to the pathogenesis of ICP and liver function damage.
【作者单位】：重庆医科大学附属第一医院产科;河北省邯郸市中心医院妇产科;
【基金】：国家自然科学基金资助项目(编号:81471473)
【分类号】：R714.25

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