非结构蛋白4B与卵巢癌免疫反应抗原域蛋白2相互作用结构域的生物信息学分析
发布时间：2019-03-29 09:20
【摘要】：目的分析非结构蛋白4B(NS4B蛋白)与卵巢癌免疫反应抗原域蛋白2(OCIAD2)相互作用的结构域。方法进入(美国国立生物技术信息中心(NCBI)主页分别查询NS4B蛋白和OCIAD2蛋白Gen Bank序列号,进入通用蛋白质数据库(Uni Prot)主页输入在NCBI中查询的NS4B蛋白和OCIAD2蛋白序列号,查询蛋白编号,进入ELM主页面,输入在Uni Prot查询到的NS4B蛋白编号A4GSN1和OCIAD2蛋白编号Q56VL3,查询跨膜结构域和线性基序。返回ELM主页,选择ELM binding domains,查询数据库里总的相互作用结构域。进入SMART主页,输入蛋白序列号或蛋白序列,查询蛋白跨膜区域和低复杂性区域。低复杂性区域的氨基酸会呈现出弹性的结构容易作为该蛋白和其他类型蛋白相结合的结构域。不同的跨膜区域因共同定位于细胞膜上可能发生相互作用。结果NS4B蛋白存在跨膜区域,OCIAD2蛋白不存在跨膜区域,没有发现NS4B和OCIAD2蛋白存在直接相互作用的线性基序。OCIAD2蛋白具有一个低复杂性区域(88~98氨基酸);NS4B蛋白具有2个低复杂性区域(106~132氨基酸和141~154氨基酸)。结论 OCIAD2蛋白与NS4B蛋白可能通过低复杂性区域发生直接相互作用。
[Abstract]:Aim to analyze the domain of interaction between non-structural protein 4B (NS4B) and protein 2 (OCIAD2) of immune response domain in ovarian cancer. Methods Gen Bank sequence numbers of NS4B protein and OCIAD2 protein were queried on the (NCBI) home page of National Biotechnology Information Center, and the sequence numbers of NS4B protein and OCIAD2 protein queried in NCBI were inputted into the (Uni Prot) home page of the general protein database. Query the protein number, go to the ELM main page, enter the NS4B protein number A4GSN1 and OCIAD2 protein number Q56VL3 that were queried in Uni Prot, and query the cross-membrane domain and linear motif. Return to the ELM home page and select the total interaction domain in the ELM binding domains, query database. Go to the SMART home page, enter the protein sequence number or protein sequence, query the protein transmembrane region and low complexity region. Amino acids in the low-complexity region present elastic structures that are easy to act as domain binding between the protein and other types of proteins. Different transmembrane regions may interact with each other because they are co-located on the cell membrane. Results there was no transmembrane region in NS4B protein and no transmembrane region in OCIAD2 protein. There was no linear motif of direct interaction between NS4B and OCIAD2 protein. OCIAD2 protein had a low complexity region (88 ~ 98 amino acids). NS4B protein has two low-complexity regions (106, 132 amino acids and 141 ~ (1) ~ (154) amino acids. Conclusion OCIAD2 protein and NS4B protein may interact directly through low complexity region.
【作者单位】： 江西农业大学生物科学与工程学院;南昌大学生命科学院人类衰老研究所;
【基金】：国家自然科学基金项目(31460667) 江西省重点研发计划项目(20161BBF60084)
【分类号】：R737.31
，
本文编号：2449384
[Abstract]:Aim to analyze the domain of interaction between non-structural protein 4B (NS4B) and protein 2 (OCIAD2) of immune response domain in ovarian cancer. Methods Gen Bank sequence numbers of NS4B protein and OCIAD2 protein were queried on the (NCBI) home page of National Biotechnology Information Center, and the sequence numbers of NS4B protein and OCIAD2 protein queried in NCBI were inputted into the (Uni Prot) home page of the general protein database. Query the protein number, go to the ELM main page, enter the NS4B protein number A4GSN1 and OCIAD2 protein number Q56VL3 that were queried in Uni Prot, and query the cross-membrane domain and linear motif. Return to the ELM home page and select the total interaction domain in the ELM binding domains, query database. Go to the SMART home page, enter the protein sequence number or protein sequence, query the protein transmembrane region and low complexity region. Amino acids in the low-complexity region present elastic structures that are easy to act as domain binding between the protein and other types of proteins. Different transmembrane regions may interact with each other because they are co-located on the cell membrane. Results there was no transmembrane region in NS4B protein and no transmembrane region in OCIAD2 protein. There was no linear motif of direct interaction between NS4B and OCIAD2 protein. OCIAD2 protein had a low complexity region (88 ~ 98 amino acids). NS4B protein has two low-complexity regions (106, 132 amino acids and 141 ~ (1) ~ (154) amino acids. Conclusion OCIAD2 protein and NS4B protein may interact directly through low complexity region.
【作者单位】： 江西农业大学生物科学与工程学院;南昌大学生命科学院人类衰老研究所;
【基金】：国家自然科学基金项目(31460667) 江西省重点研发计划项目(20161BBF60084)
【分类号】：R737.31
，
本文编号：2449384
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