子痫前期患者胎盘组织中IL-21、IL-22的表达水平及意义
发布时间：2019-04-04 12:29
【摘要】：目的:子痫前期(preeclampsia,PE)是妊娠期高血压疾病中较为常见的一种,在初次妊娠中发病率大约2%-7%[1],在发展中国家发病率更高。其以蛋白尿和高血压为主要临床表现,可同时伴有肝脏、肾脏等多脏器功能损害甚至衰竭,显著影响孕产妇、围产儿的病死率。目前关于子痫前期的发病机制尚未阐明,有学者认为其发病可能源于胎盘:母胎界面免疫耐受异常,导致胎盘功能障碍,胎盘产生多种因子进入母体血液循环进而引发各种临床症状。并且目前的研究发现,Th1/Th2/Th17/Treg平衡学说已代替之前的Th1/Th2学说。本文通过研究子痫前期患者(轻度组、重度组)与正常妊娠孕妇胎盘组织中白介素-21(IL-21)、白介素-22(IL-22)的表达水平,进而探讨二者及Th17/Treg在子痫前期发病机制中的可能作用及相互关系。方法:选取2014年4月至2014年8月收住我科(山西医科大学第一医院产科)的子痫前期患者60例(轻度子痫前期组30例,重度子痫前期组30例),正常妊娠孕妇30例(对照组)。采用ELISA方法检测子痫前期组及对照组胎盘组织中IL-21、IL-22含量。结果:1.在蛋白表达水平,IL-21、IL-22在各组中均有表达,但是实验组(轻度子痫前期组、重度子痫前期组)胎盘组织中的表达明显高于对照组,差异有统计学意义(P0.05)。2.子痫前期患者胎盘组织中IL-21的表达:子痫前期重度组IL-21表达虽然高于轻度组,但差异无统计学意义(P0.05);子痫前期患者胎盘组织中IL-22的表达:子痫前期重度组IL-22表达高于轻度组,但差异无统计学意义(P0.05)。3.子痫前期组胎盘组织中IL-21和IL-22的相关性分析:子痫前期患者胎盘中的IL-21水平与IL-22水平呈正相关,有统计学意义(r=0.803,p0.001)。结论:1.子痫前期组胎盘组织中IL-21、IL-22的含量与对照组相比均升高,推断IL-21、IL-22可能与子痫前期的发病有关,进而提示Th17/Treg可能参与子痫前期的发生。2.子痫前期患者胎盘组织中IL-21水平与IL-22水平呈正相关,提示IL-21、IL-22在子痫前期患者胎盘组织中可能存在相关性。
[Abstract]:Aim: preeclampsia (preeclampsia,PE) is one of the most common hypertensive disorder complicating pregnancy. The incidence of preeclampsia in the first pregnancy is about 2% / 7% [1], which is higher in developing countries. Albuminuria and hypertension are the main clinical manifestations, and can be accompanied by liver, kidney and other multiple organ function damage and even failure, which significantly affects the mortality of pregnant women and perinatal infants. At present, the pathogenesis of pre-eclampsia has not been clarified, some scholars believe that its pathogenesis may be due to placenta: maternal-fetal interface immune tolerance, resulting in placental dysfunction. Placenta produces a variety of factors into the maternal blood circulation, leading to a variety of clinical symptoms. And the current study found that the Th1/Th2/Th17/Treg equilibrium theory has replaced the previous Th1/Th2 theory. The expression of interleukin-21 (IL-21) and interleukin-22 (IL-22) in placenta of preeclampsia patients (mild group, severe group) and normal pregnant women were studied. Furthermore, the possible role of Th17/Treg and their roles in the pathogenesis of preeclampsia and their relationship were discussed. Methods: from April 2014 to August 2014, 60 cases of preeclampsia (30 cases in mild preeclampsia group, 30 cases in severe preeclampsia group) and 30 normal pregnant women (control group) were enrolled in our department (first Hospital of Shanxi Medical University). The content of IL-21,IL-22 in placenta of preeclampsia group and control group was detected by ELISA. Results: 1. At the level of protein expression, IL-21,IL-22 was expressed in all groups, but the expression in placenta of the experimental group (mild preeclampsia group, severe preeclampsia group) was significantly higher than that of the control group. The difference was statistically significant (P0.05). 2. The expression of IL-21 in placenta of preeclampsia patients: the expression of IL-21 in severe preeclampsia group was higher than that in mild preeclampsia group, but there was no significant difference (P0.05). The expression of IL-22 in placenta of preeclampsia patients: the expression of IL-22 in severe preeclampsia group was higher than that in mild preeclampsia group, but there was no significant difference (P0.05). Correlation analysis of IL-21 and IL-22 in placenta of preeclampsia group: the level of IL-21 in placenta of preeclampsia patients was positively correlated with the level of IL-22 (r = 0.803, p0.001). Conclusions: 1. The content of IL-21,IL-22 in placenta of preeclampsia group was higher than that of control group, which suggested that IL-21,IL-22 might be related to the pathogenesis of preeclampsia, and that Th17/Treg might be involved in the occurrence of preeclampsia. 2. There was a positive correlation between the levels of IL-21 and IL-22 in placenta of patients with preeclampsia, which suggested that IL-21,IL-22 might be correlated in placental tissues of patients with preeclampsia.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R714.244
本文编号：2453810
[Abstract]:Aim: preeclampsia (preeclampsia,PE) is one of the most common hypertensive disorder complicating pregnancy. The incidence of preeclampsia in the first pregnancy is about 2% / 7% [1], which is higher in developing countries. Albuminuria and hypertension are the main clinical manifestations, and can be accompanied by liver, kidney and other multiple organ function damage and even failure, which significantly affects the mortality of pregnant women and perinatal infants. At present, the pathogenesis of pre-eclampsia has not been clarified, some scholars believe that its pathogenesis may be due to placenta: maternal-fetal interface immune tolerance, resulting in placental dysfunction. Placenta produces a variety of factors into the maternal blood circulation, leading to a variety of clinical symptoms. And the current study found that the Th1/Th2/Th17/Treg equilibrium theory has replaced the previous Th1/Th2 theory. The expression of interleukin-21 (IL-21) and interleukin-22 (IL-22) in placenta of preeclampsia patients (mild group, severe group) and normal pregnant women were studied. Furthermore, the possible role of Th17/Treg and their roles in the pathogenesis of preeclampsia and their relationship were discussed. Methods: from April 2014 to August 2014, 60 cases of preeclampsia (30 cases in mild preeclampsia group, 30 cases in severe preeclampsia group) and 30 normal pregnant women (control group) were enrolled in our department (first Hospital of Shanxi Medical University). The content of IL-21,IL-22 in placenta of preeclampsia group and control group was detected by ELISA. Results: 1. At the level of protein expression, IL-21,IL-22 was expressed in all groups, but the expression in placenta of the experimental group (mild preeclampsia group, severe preeclampsia group) was significantly higher than that of the control group. The difference was statistically significant (P0.05). 2. The expression of IL-21 in placenta of preeclampsia patients: the expression of IL-21 in severe preeclampsia group was higher than that in mild preeclampsia group, but there was no significant difference (P0.05). The expression of IL-22 in placenta of preeclampsia patients: the expression of IL-22 in severe preeclampsia group was higher than that in mild preeclampsia group, but there was no significant difference (P0.05). Correlation analysis of IL-21 and IL-22 in placenta of preeclampsia group: the level of IL-21 in placenta of preeclampsia patients was positively correlated with the level of IL-22 (r = 0.803, p0.001). Conclusions: 1. The content of IL-21,IL-22 in placenta of preeclampsia group was higher than that of control group, which suggested that IL-21,IL-22 might be related to the pathogenesis of preeclampsia, and that Th17/Treg might be involved in the occurrence of preeclampsia. 2. There was a positive correlation between the levels of IL-21 and IL-22 in placenta of patients with preeclampsia, which suggested that IL-21,IL-22 might be correlated in placental tissues of patients with preeclampsia.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R714.244
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