CXXC指蛋白5在上皮性卵巢癌中的表达及其临床意义

发布时间：2019-04-11 16:49

【摘要】：背景与目的:上皮性卵巢癌是最常见的卵巢肿瘤类型,发病率高,治疗效果不满意,生存率低。CXXC指蛋白5(CXXC finger protein 5,CXXC5)在上皮性卵巢癌中的研究鲜见报道,该研究通过对CXXC5在上皮性卵巢癌中的表达和对上皮性卵巢癌细胞功能的研究,旨在探讨CXXC5在上皮性卵巢癌中可能的作用和临床意义。方法:1通过肿瘤基因图集(The Cancer Genoma Atlas,TCGA)数据库提供的数据分析CXXC5在上皮性卵巢癌基因组中的变异;2通过免疫组织化学法(immunohistochemistry,IHC)检测CXXC5在上皮性卵巢癌组织芯片中的表达情况,并分析CXXC5的表达与临床病理特征之间的关系;3通过蛋白[质]印迹法(Western blot)和实时定量PCR(real-time quantitative PCR,q RT-PCR)检测CXXC5在5株上皮性卵巢癌细胞系中的表达情况,选取表达量最高的ES-2细胞株;4使用慢病毒包装质粒转染ES-2细胞株,经嘌呤霉素筛选后构建稳定转染干扰细胞株;使用细胞计数试剂盒(cell counting kit-8,CCK8)检测细胞增殖能力的变化。结果:1CXXC5在TCGA提供的上皮性卵巢癌基因组中以表达为主;2CXXC5在上皮性卵巢和卵巢良性上皮性囊肿中的高表达率分别为39.3%和13.5%,差异有统计学意义(P=0.003);CXXC5在浆液性卵巢癌、黏液性卵巢癌、子宫内膜样卵巢癌和卵巢透明细胞癌中的高表达率分别为43.0%、22.9%、23.5%和66.7%,两两比较差异均有统计学意义(P均=0.014);CXXC5在有淋巴结转移和无淋巴结转移的上皮性卵巢癌中表达率为60.0%和35.8%,差异有统计学意义(P=0.022);3CXXC5稳定干扰后,卵巢癌透明细胞株ES-2的增殖能力明显减弱,差异有统计学意义(P0.05)。结论:CXXC5基因可能有促进卵巢癌细胞增殖的作用,可能是上皮性卵巢癌预后不良的生物标志物。
[Abstract]:Background & objective: epithelial ovarian cancer is the most common type of ovarian cancer with high incidence, unsatisfactory therapeutic effect and low survival rate. CXXC finger protein 5 (CXXC finger protein 5, CXXC5) is rarely reported in epithelial ovarian cancer. The purpose of this study was to explore the possible role and clinical significance of CXXC5 in epithelial ovarian cancer by studying the expression of CXXC5 in epithelial ovarian cancer and the function of epithelial ovarian cancer cells. Methods: (1) to analyze the variation of CXXC5 in the genome of epithelial ovarian cancer (EOC) by the data provided by (The Cancer Genoma Atlas,TCGA (tumor gene atlas) database; (2) Immunohistochemistry (immunohistochemistry,IHC) was used to detect the expression of CXXC5 in the tissue microarray of epithelial ovarian carcinoma, and the relationship between the expression of CXXC5 and clinicopathological features was analyzed. 3The expression of CXXC5 in 5 epithelial ovarian cancer cell lines was detected by protein blot (Western blot) and real-time quantitative PCR (real-time quantitative PCR,q RT-PCR). The ES-2 cell line with the highest expression level was selected. (4) ES-2 cell line was transfected with lentivirus packaging plasmid and then stably transfected with puromycin. Cell counting kit (cell counting kit-8,CCK8) was used to detect the change of cell proliferation. Results: the high expression rate of 1CXXC5 was 39.3% in epithelial ovarian carcinoma and 13.5% in benign epithelial ovarian cyst, and the expression rate of 2CXXC5 in epithelial ovarian carcinoma and benign ovarian cyst was 39.3% and 13.5%, respectively, with significant difference (P < 0.05). The high expression rate of CXXC5 in serous ovarian cancer, mucinous ovarian cancer, endometrial ovarian carcinoma and ovarian clear cell carcinoma was 43.0%, 22.9%, 23.5% and 66.7%, respectively. There was significant difference between the two groups (P = 0.014). The expression rate of CXXC5 in epithelial ovarian carcinoma with and without lymph node metastasis was 60.0% and 35.8% respectively, and the difference was statistically significant (P < 0.05). After stable interference of 3CXXC5, the proliferation ability of transparent ovarian cancer cell line ES-2 was significantly decreased (P0.05). Conclusion: CXXC5 gene may promote the proliferation of ovarian cancer cells and may be a biomarker of poor prognosis of epithelial ovarian cancer.
【作者单位】：南方医科大学第三临床医学院;南方医科大学附属奉贤医院妇产科;常州市妇幼保健院妇产科;南方医科大学附属奉贤医院病理科;
【分类号】：R737.31

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