叉头框F2在宫腔粘连中的表达及意义

发布时间：2019-04-16 18:59

【摘要】：目的:建立稳定的宫腔粘连细胞模型,检测叉头框F2(Fox F2)表达。方法:原代培养人子宫内膜基质细胞(HESCs),免疫荧光法进行细胞鉴定。用0、2.5、5和10ng/ml转化生长因子β1(TGF-β1)作用于HESCs 48h,10ng/ml TGF-β1作用于HESCs 12、24、48h和72h,PCR及Western blot法检测α-平滑肌肌动蛋白(α-SMA)、胶原I(COLI)及Fox F2 m RNA和蛋白表达。结果:与0ng/ml组比较,2.5、5ng/ml和10ng/ml组α-SMA、COLI及Fox F2的m RNA和蛋白表达均逐渐增加。随着10ng/ml TGF-β1作用时间的延长,与12h组相比,24、48和72h组α-SMA、COLI和Fox F2的m RNA和蛋白表达逐渐增加。结论:TGF-β1可诱导HESCs发生纤维化改变,建立宫腔粘连细胞模型。随着TGF-β1作用时间的延长,作用浓度的增加,Fox F2在宫腔粘连中表达增加。
[Abstract]:Aim: to establish a stable model of uterine cavity adhesion cells and detect the expression of Fox F2. Methods: primary cultured human endometrial stromal cells were identified by (HESCs), immunofluorescence assay. The HESCs was treated with 0, 2.5, 5 and 10ng/ml transforming growth factor 尾 1 (TGF- 尾 1) for 48 h, and 10 ng / ml TGF- 尾 1 was treated with HESCs 12, 24, 48 h and 72 h. The 伪-SMA), was detected by Western blot. The expression of collagen I (COLI) and Fox F 2m RNA and protein were observed. Results: compared with 0ng/ml group, the mRNA and protein expression of 伪-SMA,COLI and Fox F2 in 2.5 ng / ml, 5 ng / ml and 10ng/ml groups increased gradually. Compared with 12h group, the expression of 伪-SMA,COLI and Fox F2 mRNA and protein in 24, 48 and 72 h groups increased gradually with the prolongation of 10ng/ml TGF- 尾 1 treatment time. Conclusion: TGF- 尾 1 can induce fibrosis changes of HESCs and establish uterine adhesion cell model. With the prolongation of TGF- 尾 1 treatment time and the increase of concentration, the expression of Fox F2 in uterine cavity adhesion increased.
【作者单位】：南方医科大学珠江医院妇产科;
【基金】：国家自然科学基金资助(No:81270658)
【分类号】：R711.74

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