不同药物载体PEDF靶向给药对子宫内膜异位病灶生长及新生血管形成相关因子的影响

发布时间：2019-04-20 07:59

【摘要】：目的在大鼠模型基础上,观察PEDF靶向给药对子宫内膜异位病灶生长及新生血管形成相关因子的影响,并比较不同药物载体的效果。方法采用自体内膜移植法建立子宫内膜异位症大鼠模型,并按计算机随机数字法分为5组,根据干预措施的不同分别为:对照组、无载体组、脂质体组、纳米脂质体组及免疫纳米脂质体组。术后4周打开大鼠腹腔,观察并测量子宫内膜异位病灶的体积,取新鲜病灶组织,采用半定量RT-PCR法检测血管内皮生长因子(VEGF)和凋亡相关因子配体(FAS-L)mRNA的相对表达量。结果无载体组、脂质体组、纳米脂质体组异位病灶体积测量结果与对照组比较差异无统计学意义(P0.05),而免疫纳米脂质体组的病灶体积均小于以上4组,差异有统计学意义(P0.05)。对照组、无载体组、脂质体组、纳米脂质体组及免疫纳米脂质体组异位病灶中VEGF mRNA的相对表达量依次降低,各组间差异有统计学意义(P0.05);对照组、无载体组、脂质体组、纳米脂质体组及免疫纳米脂质体组异位病灶中Fas-L的表达水平依次升高,各组间差异有统计学意义(P0.05)。结论添加外源性PEDF能够降低子宫内膜异位病灶中VEGF的表达,并促进Fas-L的表达,对新生血管形成及异位病灶生长产生抑制作用。在各种给药载体系统中,以特异性靶向性的免疫纳米脂质体效果最佳。
[Abstract]:Aim to observe the effect of targeted administration of PEDF on the growth of endometriosis and neovascularization on the basis of rat model, and to compare the effects of different drug carriers on the growth of endometriosis and neovascularization. Methods the rat model of endometriosis was established by autologous membrane transplantation and divided into 5 groups according to the computer random number method. According to the different intervention measures, the rats were divided into three groups: control group, vector-free group, liposome group, and the control group, the carrier-free group, the liposome group, and the control group. Nanoliposomes and immuno-nanoliposomes. Four weeks after operation, the abdominal cavity of rats was opened, the volume of endometriosis lesion was observed and measured, and fresh lesion tissue was obtained. The relative expression levels of vascular endothelial growth factor (VEGF) and apoptosis-related factor ligand (FAS-L) mRNA were detected by semi-quantitative RT-PCR. Results there was no significant difference between the non-carrier group, the liposome group and the nano-liposomes group in the measurement of ectopic lesion volume (P0.05), but the focus volume in the immuno-nano-liposome group was smaller than that in the above four groups. The difference was statistically significant (P0.05). The relative expression of VEGF mRNA in ectopic lesions of control group, carrier-free group, liposome group, nano-liposomes group and immuno-nanoliposome group decreased in turn, and there was significant difference among the three groups (P0.05). The expression level of Fas-L in ectopic lesions of control group, carrier-free group, liposome group, nano-liposomes group and immuno-nanoliposome group was increased in turn, and there was significant difference among the three groups (P0.05). Conclusion exogenous PEDF can decrease the expression of VEGF and promote the expression of Fas-L in endometriotic lesions, and inhibit angiogenesis and ectopic lesion growth. Among all kinds of drug delivery carrier systems, immuno-nanoliposomes with specific targeting were the best.
【作者单位】：信阳职业技术学院;信阳市中心医院妇产科;信阳职业技术学院附属医院血液透析科;
【分类号】：R711.71

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