抑癌基因PTEN对子宫内膜异位症发生及血管生成的影响

发布时间：2019-04-22 07:40

【摘要】：目的:通过体外细胞实验及子宫内膜异位症动物模型研究磷酸酶基因(PTEN)蛋白对子宫内膜异位症(EMs)血管生成的影响。方法:利用慢病毒载体分别在原代子宫内膜细胞中过表达和静默PTEN表达。Matrigel胶三维培养观察不同PTEN表达组细胞血管生长拟态。利用SCID鼠构建人鼠嵌合子宫内膜异位症模型,HE及免疫组化技术检测不同PTEN表达组人鼠嵌合子宫内膜异位症模型小鼠异位内膜组织的病理改变及VEGF蛋白表达。结果:与PTEN高表达组相比,PTEN低表达组的子宫内膜细胞更易在Matrigel胶上形成血管拟态,血管生成明显增加;PTEN低表达的子宫内膜组织更易在SCID小鼠体内形成异位病灶,VEGF表达相对较高。结论:PTEN通过调节血管生成对EMs的发生发展有一定的抑制作用,推测PTEN基因有用于治疗EMs的可能。
[Abstract]:Aim: to study the effect of phosphatase gene (PTEN) protein on angiogenesis of (EMs) in endometriosis by cell experiment in vitro and animal model of endometriosis. Methods: lentiviral vector was used to over-express and silent PTEN expression in primary endometrial cells, and the vascular growth mimicry of different PTEN expression groups was observed in three-dimensional culture with Matrigel gel. The human mouse chimeric endometriosis model was established by SCID mice. The pathological changes and VEGF protein expression of human mouse chimeric endometriosis model mice with different PTEN expression groups were detected by HE and immunohistochemistry. Results: compared with the high expression group of PTEN, the endometrial cells in the low expression group of PTEN were more likely to form vascular mimicry on Matrigel glue, and the angiogenesis was significantly increased. The endometrial tissue with low expression of PTEN was more likely to form ectopic lesions in SCID mice, and the expression of VEGF was relatively high. Conclusion: PTEN may inhibit the occurrence and development of EMs by regulating angiogenesis, suggesting that PTEN gene may be used in the treatment of EMs.
【作者单位】：南京医科大学附属南京妇幼保健院妇科;
【基金】：南京市医学科技发展基金项目(No:YKK12104) 南京医科大学科技发展基金项目(No:2011NJMU213)
【分类号】：R711.71

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