KLOTHO与Wnt10b在子宫颈鳞状细胞癌中的表达及其相关性研究
发布时间：2019-04-26 02:11
【摘要】：目的： 通过在正常宫颈上皮组织、宫颈上皮内瘤变(cervical intraepithelial neoplasia, CIN)以及宫颈鳞状细胞癌(cervical squamous cell carcinoma, CSCC)中检测Klotho和Wnt10b表达,旨在探索宫颈癌发生、发展过程中两者所发挥的作用及二者之间的相关性。 方法： (1)采用免疫组化SP法检测Klotho及Wnt10b在20例正常宫颈上皮组织、41例CIN组织及106例CSCC组织中的表达。 (2)使用实时荧光定量法(real-time PCR;RT-PCR)检测5例正常宫颈组织、3例CIN组织以及7例CSCC组织中Klotho与Wnt10b的mRNA含量。 结果： (1)Klotho在正常宫颈上皮组织中的表达明显高于CIN组织及CSCC组织,其在正常宫颈上皮组织、CIN组织及CSCC组织中的阳性表达率依次减低,差异有统计学意义(P0.05)。Wnt10b在CSCC组织中的表达明显高于正常宫颈组织、CIN组织,其在正常宫颈上皮组织、CIN组织及CSCC组织中的阳性表达率依次上调,差异有统计学意义(P0.05) (2) Klotho表达与宫颈鳞癌患者年龄无相关性(P0.05),与宫颈癌FIGO分期、组织学分化程度以及淋巴结转移有关,差异有统计学意义(P0.05)；Wnt10b表达与患者年龄无相关性(P0.05),与分化程度、FIGO分期及淋巴结转移有关,差异有统计学意义(P0.05)。 (3)Klotho mRNA在正常宫颈上皮组织中的表达显著高于CSCC组织,Wnt10b mRNA在CSCC组织中的表达显著高于正常宫颈组织。 (4)在CSCC组织中,Klotho蛋白与Wnt10b的蛋白表达呈负相关(rs=-0.416,P0.01)。 结论： (1) Klotho蛋白在正常宫颈组织、CIN组织及CSCC组织中的阳性表达率逐步降低,与宫颈鳞癌的分化程度、FIGO分期以及淋巴结转移密切相关,这表明Klotho在抑制宫颈癌的发生发展过程中发挥重要作用。 (2) Wnt10b在正常宫颈组织、CIN及CSCC组织中的阳性表达率依次增高,且与宫颈鳞癌的分化程度、FIGO分期以及淋巴结转移等具有相关性,提示Wnt10b在宫颈鳞癌的发生、发展、侵袭以及转移中有着重要的作用。 (3)与正常宫颈粘膜组相比较,CSCC组织中Klotho mRNA含量减少,而Wnt10b mRNA含量增多,并且Klotho与Wnt10b蛋白表达呈负相关,提示Klotho和Wnt10b可能在宫颈癌的发生、发展过程中起重要作用,联合检测Klotho和Wnt10b有助于宫颈癌的早期诊断。
[Abstract]:Objective: to detect the expression of Klotho and Wnt10b in normal cervical epithelium, cervical intraepithelial neoplasia (cervical intraepithelial neoplasia, CIN) and cervical squamous cell carcinoma (cervical squamous cell carcinoma, CSCC) in order to explore the carcinogenesis of cervical cancer. The role of the two in the process of development and the correlation between the two. Methods: (1) Immunohistochemistry SP method was used to detect the expression of Klotho and Wnt10b in 20 cases of normal cervical epithelium, 41 cases of CIN and 106 cases of CSCC. (2) the mRNA contents of Klotho and Wnt10b in 5 cases of normal cervical tissues, 3 cases of CIN tissues and 7 cases of CSCC tissues were detected by real-time fluorescence quantitative analysis (real-time PCR;RT-PCR). Results: (1) the expression of Klotho in normal cervical epithelial tissues was significantly higher than that in CIN and CSCC tissues, and the positive expression rates in normal cervical epithelial tissues, CIN tissues and CSCC tissues decreased in turn. The expression of Wnt10b in CSCC tissue was significantly higher than that in normal cervical tissue, CIN tissue, and the positive rate of WNT 10b in normal cervical epithelial tissue, CIN tissue and CSCC tissue was up-regulated in turn, and the expression of Wnt10b in normal cervical epithelial tissue, CIN tissue and CSCC tissue was up-regulated. The difference was statistically significant (P0.05) (2) the expression of Klotho was not correlated with the age of cervical squamous cell carcinoma (P0.05), but correlated with FIGO stage, histological differentiation and lymph node metastasis of cervical cancer. The difference was statistically significant (P0.05); The expression of Wnt10b was not correlated with age (P0.05), but correlated with the degree of differentiation, FIGO stage and lymph node metastasis (P0.05). (3) the expression of) Klotho mRNA in normal cervical epithelial tissues was significantly higher than that in CSCC tissues, and the expression of Wnt10b mRNA in CSCC tissues was significantly higher than that in normal cervical tissues. (4) in CSCC tissues, the expression of Klotho protein was negatively correlated with the expression of Wnt10b protein (rs=-0.416,P0.01). Conclusion: (1) the positive expression rate of Klotho protein in normal cervical tissues, CIN tissues and CSCC tissues decreased gradually, which was closely related to the differentiation degree, FIGO stage and lymph node metastasis of cervical squamous cell carcinoma. These results suggest that Klotho plays an important role in inhibiting the occurrence and development of cervical cancer. (2) the positive expression rate of Wnt10b in normal cervical tissues, CIN and CSCC tissues was increased in turn, and it was correlated with the differentiation degree, FIGO stage and lymph node metastasis of cervical squamous cell carcinoma, which suggested the occurrence and development of Wnt10b in cervical squamous cell carcinoma. Invasion and metastasis play an important role. (3) compared with the normal cervical mucosa group, the content of Klotho mRNA in CSCC tissue decreased, while the content of Wnt10b mRNA increased, and the expression of Klotho was negatively correlated with the expression of Wnt10b protein, suggesting that Klotho and Wnt10b might play an important role in the development of cervical cancer. The combined detection of Klotho and Wnt10b is helpful for the early diagnosis of cervical cancer.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.33
本文编号：2465665
[Abstract]:Objective: to detect the expression of Klotho and Wnt10b in normal cervical epithelium, cervical intraepithelial neoplasia (cervical intraepithelial neoplasia, CIN) and cervical squamous cell carcinoma (cervical squamous cell carcinoma, CSCC) in order to explore the carcinogenesis of cervical cancer. The role of the two in the process of development and the correlation between the two. Methods: (1) Immunohistochemistry SP method was used to detect the expression of Klotho and Wnt10b in 20 cases of normal cervical epithelium, 41 cases of CIN and 106 cases of CSCC. (2) the mRNA contents of Klotho and Wnt10b in 5 cases of normal cervical tissues, 3 cases of CIN tissues and 7 cases of CSCC tissues were detected by real-time fluorescence quantitative analysis (real-time PCR;RT-PCR). Results: (1) the expression of Klotho in normal cervical epithelial tissues was significantly higher than that in CIN and CSCC tissues, and the positive expression rates in normal cervical epithelial tissues, CIN tissues and CSCC tissues decreased in turn. The expression of Wnt10b in CSCC tissue was significantly higher than that in normal cervical tissue, CIN tissue, and the positive rate of WNT 10b in normal cervical epithelial tissue, CIN tissue and CSCC tissue was up-regulated in turn, and the expression of Wnt10b in normal cervical epithelial tissue, CIN tissue and CSCC tissue was up-regulated. The difference was statistically significant (P0.05) (2) the expression of Klotho was not correlated with the age of cervical squamous cell carcinoma (P0.05), but correlated with FIGO stage, histological differentiation and lymph node metastasis of cervical cancer. The difference was statistically significant (P0.05); The expression of Wnt10b was not correlated with age (P0.05), but correlated with the degree of differentiation, FIGO stage and lymph node metastasis (P0.05). (3) the expression of) Klotho mRNA in normal cervical epithelial tissues was significantly higher than that in CSCC tissues, and the expression of Wnt10b mRNA in CSCC tissues was significantly higher than that in normal cervical tissues. (4) in CSCC tissues, the expression of Klotho protein was negatively correlated with the expression of Wnt10b protein (rs=-0.416,P0.01). Conclusion: (1) the positive expression rate of Klotho protein in normal cervical tissues, CIN tissues and CSCC tissues decreased gradually, which was closely related to the differentiation degree, FIGO stage and lymph node metastasis of cervical squamous cell carcinoma. These results suggest that Klotho plays an important role in inhibiting the occurrence and development of cervical cancer. (2) the positive expression rate of Wnt10b in normal cervical tissues, CIN and CSCC tissues was increased in turn, and it was correlated with the differentiation degree, FIGO stage and lymph node metastasis of cervical squamous cell carcinoma, which suggested the occurrence and development of Wnt10b in cervical squamous cell carcinoma. Invasion and metastasis play an important role. (3) compared with the normal cervical mucosa group, the content of Klotho mRNA in CSCC tissue decreased, while the content of Wnt10b mRNA increased, and the expression of Klotho was negatively correlated with the expression of Wnt10b protein, suggesting that Klotho and Wnt10b might play an important role in the development of cervical cancer. The combined detection of Klotho and Wnt10b is helpful for the early diagnosis of cervical cancer.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.33
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