低氧培养对未成熟卵体外成熟及胚胎发育潜能的影响

发布时间：2019-05-11 08:08

【摘要】：目的:研究辅助生殖技术超促排卵治疗中获得的未成熟卵在低氧培养体系中的发育潜能。方法:卵胞质内单精子显微注射(ICSI)或体外受精-胚胎移植中(IVF-ET)短时受精中挑选出的GV(germinal vesicle)期卵母细胞80枚,MI(metaphase I)期卵母细胞124枚,随机分为实验组和对照组,实验组置于低氧分压(体积分数5%)的三气培养箱中、对照组置于高氧分压(体积分数20%)常规培养箱中进行体外成熟培养(IVM)及随后的囊胚和胚胎培养。IVM后成熟的卵母细胞进行ICSI受精,观察受精率、卵裂率及囊胚形成率。结果:实验组GV期未成熟卵体外培养至MII卵的成熟率、双原核(2PN)率、卵裂率、囊胚形成率及优质囊胚率与对照组无统计学差异(P0.05)。但实验组MI期卵培养至MII卵的成熟率[96.8%(60/62)]、2PN率[80%(48/60)]、囊胚形成率[43.8%(21/48)]及优质囊胚率[20.8%(10/48)]均高于对照组[分别为58.1%(36/62),52.8%(19/36),11.8%(2/17)和0.0%(0/17)],差异有统计学意义(P0.05),而2种培养体系的卵裂率无统计学差异。结论:超促排卵中获得的未成熟卵,特别是MI期裸卵在低氧培养体系中进行体外成熟及后续的胚胎培养,有利于获得优质囊胚。
[Abstract]:Aim: to study the developmental potential of immature eggs obtained from superovulatory therapy with assisted reproductive technology in hypoxia culture system. Methods: 80 GV (germinal vesicle) stage oocytes were selected during in vitro fertilization-embryo transfer (IVF-ET) by intracytoplasmic sperm microinjection of (ICSI) or in vitro fertilization-embryo transfer (IVF-ET). The experimental group was randomly divided into experimental group and control group. The experimental group was placed in a three-gas incubator with hypoxia partial pressure (5% volume fraction). (IVM), blastocysts and embryos were cultured in vitro and then blastocysts and embryos were cultured in normal culture box with hyperoxic partial pressure (20% by volume). The fertilization rate, cleavage rate and blastocyst formation rate of mature oocytes were observed by ICSI fertilization after IVV.The fertilization rate, cleavage rate and blastocyst formation rate were observed. Results: there was no significant difference in maturation rate, double prokaryotic (2PN) rate, cleavage rate, blastocyst formation rate and high quality blastocyst rate between the experimental group and the control group (P0.05). There was no significant difference between the experimental group and the control group (P0.05). But the maturation rate from MI stage to MII stage [96.8% (60 / 62)] and the 2PN rate [80% (48 / 60)] in the experimental group. The blastocyst formation rate [43.8% (21 / 48)] and the quality blastocyst rate [20.8% (10 / 48)] were higher than those in the control group [58.1% (36 / 62), 52.8% (19 / 36), respectively]. 11.8% (2 / 17) and 0.0% (0 / 17), the difference was statistically significant (P0.05), but there was no significant difference in cleavage rate between the two culture systems. Conclusion: in vitro maturation and subsequent embryo culture of immature eggs, especially MI stage bare eggs, in hypoxia culture system are beneficial to obtaining high quality blastocysts.
【作者单位】：广州医科大学第三附属医院生殖医学中心广东省生殖医学重点实验室;
【基金】：市科信局项目(2011J4100047) 国家自然科学基金资助项目(81401504) 广州市教育局资助项目(1201430205)
【分类号】：R714.8

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