蟾蜍灵对宫颈癌细胞的增殖抑制作用及其机制研究

发布时间：2019-05-19 17:43

【摘要】：背景与目的:宫颈癌仍然是妇科肿瘤致死病因的第二位,部分原因为化疗耐药。蟾蜍灵是传统中药蟾酥的成分之一,目前在国内广泛应用于肿瘤的治疗中。该研究旨在探讨蟾蜍灵对宫颈癌细胞ME180和C33A的增殖抑制作用及相关机制。方法:采用CCK8(cell counting kit-8)法检测蟾蜍灵对宫颈癌细胞增殖的抑制作用,采用葡萄糖检测分析试剂盒(glucose assay kit)进行细胞内糖代谢检测分析,采用实时荧光定量聚合酶链反应(quantitative real-time polymerase chain reaction,q RT-PCR)检测细胞内葡萄糖转运蛋白1(glucose transporter1,GLUT1)和己糖激酶2(hexokinase 2,HK2)的m RNA的表达水平,采用蛋白[质]印迹法(Western blot)检测原癌基因C-MYC和缺氧诱导因子1α(Hif-1α)表达的变化。结果:CCK8法结果显示,蟾蜍灵能明显抑制宫颈癌细胞ME180和C33A细胞增殖(P=0.027,P=0.018)。糖代谢检测结果显示,蟾蜍灵处理组葡萄糖代谢水平显著降低(P=0.034,P=0.036)。q RT-PCR检测结果显示,蟾蜍灵处理组糖代谢相关指标GLUT1(P=0.019,P=0.016)和HK2(P=0.039,P=0.041)的表达水平明显下调。Western blot检测结果显示,蟾蜍灵处理后宫颈癌细胞内C-MYC和Hif-1α蛋白表达水平显著下调。结论:蟾蜍灵可以通过降低宫颈癌细胞ME180和C33A糖代谢水平,从而抑制细胞增殖。
[Abstract]:Background & objective: cervical cancer is still the second leading cause of death in gynecological tumors, partly due to chemotherapy resistance. Bufalin is one of the components of traditional Chinese medicine toad pastry, which is widely used in the treatment of tumor in China. The purpose of this study was to investigate the inhibitory effect of bufalin on the proliferation of cervical cancer cells ME180 and C33A and its related mechanism. Methods: CCK8 (cell counting kit-8 assay was used to detect the inhibitory effect of bufalin on the proliferation of cervical cancer cells, and glucose assay kit (glucose assay kit) was used to detect intracellular glucose metabolism. The expression of m RNA in intracellular glucose transporter 1 (glucose transporter1,GLUT1) and hexokinase 2 (hexokinase 2, HK2) was detected by real time fluorescence quantitative polymerase chain reaction (quantitative real-time polymerase chain reaction,q RT-PCR). The expression of proto-Oncogene C-MYC and hypoxia inducible factor 1 伪 (Hif-1 伪) was detected by (Western blot). Results: CCK8 assay showed that toad could significantly inhibit the proliferation of cervical cancer cells ME180 and C33A cells (P 鈮，

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