miR-21、miR-27a、miR-27b在肥胖及代谢紊乱型多囊卵巢综合征患者淋巴细胞中的表达

发布时间：2019-05-30 07:43

【摘要】：背景:多囊卵巢综合征(polycystic ovary syndrome,PCOS)是一种常见的内分泌、代谢紊乱疾病,多伴有肥胖表型。其发病机制尚不明确,当前认为是由遗传、环境等多因素多环节共同引起。Micro RNA(miRNA)作为一种非编码小分子单链RNA,主要在转录后水平调控基因的表达。miRNA的异常表达与多种代谢紊乱有关,包括肥胖、糖尿病,以及PCOS。近来许多学者发现miR-21、miR-27a、miR-27b与炎症、激素代谢、脂肪合成、肥胖、胰岛素信号传导有关,然而目前关于miR-21、miR-27a、miR-27b的表达与PCOS患者肥胖及相关代谢紊乱的关系研究甚少。目的:研究肥胖和非肥胖型PCOS患者外周血淋巴细胞miR-21、miR-27a、miR-27b的表达水平及控制体重对其表达的影响,探讨miR-21、miR-27a、miR-27b与PCOS肥胖及相关代谢紊乱(高雄激素血症、高胰岛素血症)的关系。方法:1.纳入PCOS患者60例,根据WHO对成年人体重指数(BMI)的划分标准分为非肥胖组(BMI25kg/m2,22例)和肥胖组(BMI≥25kg/m2,38例)。2.采用电化学发光检测PCOS患者血清促卵泡素(b FSH)、促黄体生成素(b LH)、雌二醇(b E2)、孕激素(b P)、睾酮(bT)、空腹胰岛素(FINS)水平。3.采用葡萄糖氧化酶法测定PCOS患者空腹血糖(Glu)。4.应用实时荧光定量PCR(q PCR)法检测外周血淋巴细胞miR-21、miR-27a、miR-27b的表达水平。5.肥胖组控制体重后复测上述指标。结果:1.肥胖组PCOS患者bT、Glu、FINS、HOMA-IR水平均显著高于非肥胖组,且2.差异有统计学意义(P0.05)。3.肥胖组PCOS患者外周血淋巴细胞miR-21、miR-27a、miR-27b的表达水平明显高于非肥胖组(2.83±0.60 vs 1.18±0.24;2.63±0.42 vs 1.13±0.19;2.74±0.49 vs1.16±0.17;P均0.05)。4.配对样本t检验结果显示肥胖组PCOS患者控制体重后b FSH、b E2、b P的水平与控制体重前相比,差异无明显统计学意义(P0.05);BMI、b LH、bT、Glu、FINS、HOMA-IR水平较控制体重前显著降低,且差异有统计学意义(P0.05)。5.肥胖组PCOS患者控制体重后外周血淋巴细胞miR-21、miR-27a、miR-27b的表达水平较控制前明显降低(1.88±0.27 vs 2.81±0.61;2.16±0.39 vs 2.69±0.37;2.27±0.40 vs 2.72±0.47;P均0.05)。6.miR-21的表达量与bT呈正相关性(r=0.342,P=0.008),miR-27a、miR-27b的表达量与FINS呈正相关性(r=0.413,P=0.001;r=0.397,P=0.002)。结论:miR-21、miR-27a、miR-27b与PCOS肥胖及相关代谢紊乱(高雄激素血症、高胰岛素血症)息息相关。miR-21可能主要与bT代谢异常有关,而miR-27a、miR-27b可能主要与胰岛素代谢异常有关。miR-21、miR-27a、miR-27b分别通过不同途径参与了PCOS肥胖和高雄激素血症、高胰岛素血症的恶性循环。
[Abstract]:Background: polycystic ovary syndrome (polycystic ovary syndrome,PCOS) is a common endocrine and metabolic disorder with obesity phenotype. Its pathogenesis is not clear, and it is currently considered to be caused by many factors, such as heredity, environment and so on. Micro RNA (miRNA), as a kind of non-coding small molecule single chain RNA, is considered to be caused by many factors, such as heredity, environment and so on. The abnormal expression of miRNA is related to a variety of metabolic disorders, including obesity, diabetes, and PCOS.. Recently, many scholars have found that miR-21,miR-27a,miR-27b is related to inflammation, hormone metabolism, fat synthesis, obesity and insulin signal transduction. However, at present, it is about miR-21,miR-27a,. There is little research on the relationship between the expression of miR-27b and obesity and related metabolic disorders in PCOS patients. Objective: to study the expression of miR-21,miR-27a,miR-27b in peripheral blood lymphocytes of obese and non-obese PCOS patients and the effect of weight control on its expression, and to explore the effect of miR-21,miR-27a,. Relationship between miR-27b and obesity and related metabolic disorders (hyperandrogenemia, hyperinsulinemia) in PCOS. Method: 1. 60 patients with PCOS were divided into non-obese group (BMI25kg/m2,22) and obese group (BMI 鈮，

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