恐伤孕鼠所产80日龄仔鼠情志与海马区去甲肾上腺素和肾上腺素水平的相关性研究

发布时间：2019-06-06 19:26

【摘要】：目的探讨妊娠期恐伤刺激对其80日龄仔鼠情志和海马区去甲肾上腺素(NE)、肾上腺素(EP)的影响,并探讨两者的相关性。方法 2015年1月—2016年3月,选取80日龄SPF级Wistar大鼠59只(雌鼠34只,雄鼠25只),采用Morris水迷宫实验评价其认知水平。选取认知基线水平一致的30只雌鼠按照随机数字表法分为模型组和对照组,各15只,另选取认知基线水平一致且体格较强壮的15只雄鼠作为交配鼠。剩余雄鼠均作电击鼠用,剩余雌鼠备用代替不孕鼠。运用旁观电击法建立妊娠期恐伤大鼠动物模型。仔鼠延续母鼠分组,至80日龄时采用随机数字表法选取15只进行实验。采用旷场实验、糖水偏好实验、悬尾实验评价仔鼠情志;通过脑立体定位仪在右侧海马区采集脑透析液,应用高效液相色谱-电子俘获检测(HPLC-ECD)法检测NE和EP水平。结果模型组旷场实验水平得分、垂直得分,糖水偏好实验糖水消耗量、糖水偏好值均低于对照组,悬尾实验静止不动时间长于对照组(P0.05)。模型组各灌流时间点海马组织NE、EP水平均低于对照组(P0.05)。仔鼠海马组织NE水平与旷场实验水平得分、垂直得分以及糖水偏好实验糖水偏好值呈正相关,与悬尾实验静止不动时间呈负相关(P0.05)。仔鼠海马组织EP水平与旷场实验水平得分以及糖水偏好实验糖水偏好值呈正相关,与悬尾实验静止不动时间呈负相关(P0.05)。结论恐伤孕鼠可改变其80日龄仔鼠的情志及海马区NE和EP的水平,其情志改变与海马区NE和EP的水平密切相关。
[Abstract]:Objective to investigate the effect of fear injury stimulation during pregnancy on norepinephrine (NE), epinephrine (EP) in 80-day-old mice and to explore the correlation between them. Methods from January 2015 to March 2016, 59 80-day-old SPF Wistar rats (34 female and 25 male) were selected and their cognitive level was evaluated by Morris water maze test. Thirty female rats with the same cognitive baseline level were randomly divided into model group (n = 15) and control group (n = 15). Another 15 male rats with the same cognitive baseline level and strong physique were selected as mating mice. The remaining male mice were used as electric shock mice, and the remaining female mice were set aside to replace infertile mice. The animal model of fear injury during pregnancy was established by bystander electric shock. The offspring were divided into groups and 15 mice were selected by random digital table at the age of 80 days. Open field test, sugar water preference test and tail suspension test were used to evaluate the emotion of neonatal rats. Brain dialysis fluid was collected in the right hippocampus by brain stereotactic apparatus, and the levels of NE and EP were detected by high performance liquid chromatography-electron capture detection (HPLC-ECD). Results the horizontal score, vertical score, sugar water preference test sugar water consumption and sugar water preference value of the model group were lower than those of the control group, and the rest time of the tail suspension test was longer than that of the control group (P 0.05). The level of NE,EP in hippocampus of the model group was lower than that of the control group at each perfusion time point (P 0.05). The level of NE in hippocampus of neonatal rats was positively correlated with the horizontal score of open field test, vertical score and sugar water preference test, and negatively correlated with the rest time of tail suspension test (P 0.05). The level of EP in hippocampus was positively correlated with the score of open field test and the value of sugar water preference in sugar water preference test, and negatively correlated with the rest time of tail suspension test (P 0.05). Conclusion the emotion of 80-day-old pregnant rats can be changed, and the levels of NE and EP in hippocampus can be changed. The emotional changes are closely related to the levels of NE and EP in hippocampus.
【作者单位】：河南中医药大学中西医结合基础学科;
【基金】：国家自然科学基金面上项目(81373790)
【分类号】：R714

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