IGF-1、DLL4及VEGF在子宫内膜异位症中的表达及其意义

发布时间：2019-06-11 04:15

【摘要】：目的:观察IGF-1、DLL4及VEGF在子宫内膜异位症中的表达,探讨以上三种因子在EMs疾病形成与进展中的相互作用关系。方法:收集2014年1月至2016年12月期间本院妇产科收治的,经病理确诊的子宫内膜异位症(排除合并卵巢良性囊肿及子宫肌瘤、乳腺增生等内分泌疾病)患者40例的存档病理标本,其中异位内膜23例,在位内膜17例。选择同期我院因子宫脱垂行子宫切除术(排除合并子宫腺肌症及其他妇科疾病)的正常内膜标本10例。采用免疫组织化学染色SP法检测IGF-1、DLL4及VEGF在子宫内膜异位症的表达。结果:(1)IGF-1在正常内膜组织、异位症在位内膜及异位症异位内膜中表达阳性率分别为40.00%,72.59%和82.61%,与正常内膜相比,异位内膜中IGF-1表达阳性率与其在位内膜均明显增高(P0.05)。(2)DLL4在正常内膜组织、异位症在位内膜及异位症异位内膜中表达阳性率分别为50.00%,82.35%和82.61%,与在位内膜及正常内膜相比较,异位内膜中DLL4表达阳性率明显增高(P0.05)。(3)VEGF在正常子宫内膜组织、异位症在位内膜和异位内膜中表达阳性率分别为40.00%,76.47%和78.26%,与在位内膜及正常内膜相比较,异位内膜VEGF表达阳性率明显增高(P0.05)。(4)异位组及在位两组中,DLL4、IGF-1及VEGF蛋白两两之间均呈正相关(P0.05),正常组中,三种蛋白两两之间均无相关性(P0.05)。结论:(1)IGF-1、DLL4及VEGF可能在子宫内膜异位症疾病的发生发展过程中扮演着重要角色。(2)IGF-1、DLL4及VEGF在子宫内膜组织中的表达不受激素周期变化的影响。(3)在子宫内膜异位症形成过程中,IGF-1、DLL4及VEGF两两之间可能存在相互协同的作用,共同促进疾病的发生与发展。
[Abstract]:Objective: to observe the expression of IGF-1,DLL4 and VEGF in Endometriosis and to explore the interaction between the above three factors in the formation and progress of EMs. Methods: from January 2014 to December 2016, Endometriosis diagnosed pathologically was collected from obstetrics and gynaecology of our hospital (excluding benign ovarian cyst and uterine myoma. 40 patients with endocrine diseases such as breast hyperplasia), including 23 cases of ectopic intima and 17 cases of eutopic intima. Ten normal intimal specimens were selected for hysterectomy (excluding adenomyosis and other gynecological diseases) due to uterine prolapse in our hospital at the same time. The expression of IGF-1,DLL4 and VEGF in Endometriosis was detected by SP method. Results: (1) the positive rates of IGF-1 expression in normal intima, eutopic intima and ectopic intima were 40.00%, 72.59% and 82.61%, respectively, which were significantly higher than those in normal intima. The positive rates of IGF-1 expression in ectopic intima and eutopic intima were significantly higher than those in eutopic intima (P 0.05). (2). The positive rates of DLL4 expression in normal intima, eutopic intima and ectopic intima were 50.00%, 50.00%, 50.00% and 50.00%, respectively. 82.35% and 82.61%. Compared with eutopic intima and normal intima, the positive rate of DLL4 expression in ectopic intima was significantly higher than that in eutopic intima and normal intima (P 0.05). (3). The positive rate of VEGF in normal intima was significantly higher than that in eutopic intima and normal intima. The positive rates of expression in eutopic intima and ectopic intima were 40.00%, 76.47% and 78.26%, respectively, which were compared with those in eutopic intima and normal intima. The positive rate of VEGF expression in ectopic intima was significantly higher than that in eutopic group (P 0.05). (4). There was a positive correlation between DLL4,IGF-1 and VEGF protein in ectopic group and eutopic group (P 0.05). In normal group, There was no correlation between the three proteins (P 0.05). Conclusion: (1) IGF-1,DLL4 and VEGF may play an important role in the occurrence and development of Endometriosis. (2) IGF-1, The expression of DLL4 and VEGF in endometrium was not affected by the change of hormone cycle. (3) there may be a synergistic effect between IGF-1,DLL4 and VEGF in the process of Endometriosis. Jointly promote the occurrence and development of diseases.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R711.71

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