MicroRNA-18a及雌激素受体α在子宫肌瘤中的表达
发布时间：2019-06-14 05:03
【摘要】：目的检测子宫肌瘤及瘤旁正常平滑肌组织中microRNA-18a(miR-18a)及雌激素受体α(ERα)的表达差异,探讨microRNA-18a及ERα与子宫肌瘤的关系。方法用原位杂交法和免疫组化法分别检测microRNA-18a和ERα在子宫肌瘤及瘤旁正常平滑肌组织石蜡切片中的表达,并评价microRNA-18a及ERα的表达之间的关系。结果子宫肌瘤组织中microRNA-18a表达显著低于瘤旁正常平滑肌组织(P0.05),而ERα表达显著高于瘤旁正常平滑肌组织(P0.05)。子宫肌瘤组织及瘤旁正常平滑肌组织中microRNA-18a及ERα的表达量呈负相关。结论 microRNA-18a可能通过影响ERα在子宫肌瘤中的表达,从而在子宫肌瘤发生发展过程中起到重大作用;microRNA-18a可能成为治疗子宫肌瘤的新靶标。
[Abstract]:Objective to detect the expression of microRNA-18a (miR-18a) and estrogen receptor 伪 (ER 伪) in uterine myoma and adjacent normal smooth muscle tissues, and to explore the relationship between microRNA-18a and ER 伪 and uterine myoma. Methods the expression of microRNA-18a and ER 伪 in paraffin sections of uterine myoma and normal smooth muscle tissue adjacent to tumor was detected by in situ hybridization and immunohistochemistry, respectively, and the relationship between the expression of microRNA-18a and ER 伪 was evaluated. Results the expression of microRNA-18a in uterine myoma was significantly lower than that in normal smooth muscle tissue adjacent to tumor (P 0.05), while the expression of ER 伪 in uterine myoma was significantly higher than that in normal smooth muscle tissue adjacent to tumor (P 0.05). There was a negative correlation between the expression of microRNA-18a and ER 伪 in uterine myoma and normal smooth muscle tissues adjacent to the tumor. Conclusion microRNA-18a may play an important role in the occurrence and development of uterine myoma by affecting the expression of ER 伪 in uterine myoma, and microRNA-18a may be a new target for the treatment of uterine myoma.
【作者单位】： 电子科技大学附属医院四川省人民医院妇产科;电子科技大学附属医院四川省人民医院药学部;
【基金】：四川省卫生厅基金资助项目(100491,120111) 四川省科技厅基金资助项目(2014FZ0103,2015JQO027,015ZR0160) 四川省人民医院青年基金资助项目(30305030606,30305030859)
【分类号】：R737.33
，
本文编号：2499124
[Abstract]:Objective to detect the expression of microRNA-18a (miR-18a) and estrogen receptor 伪 (ER 伪) in uterine myoma and adjacent normal smooth muscle tissues, and to explore the relationship between microRNA-18a and ER 伪 and uterine myoma. Methods the expression of microRNA-18a and ER 伪 in paraffin sections of uterine myoma and normal smooth muscle tissue adjacent to tumor was detected by in situ hybridization and immunohistochemistry, respectively, and the relationship between the expression of microRNA-18a and ER 伪 was evaluated. Results the expression of microRNA-18a in uterine myoma was significantly lower than that in normal smooth muscle tissue adjacent to tumor (P 0.05), while the expression of ER 伪 in uterine myoma was significantly higher than that in normal smooth muscle tissue adjacent to tumor (P 0.05). There was a negative correlation between the expression of microRNA-18a and ER 伪 in uterine myoma and normal smooth muscle tissues adjacent to the tumor. Conclusion microRNA-18a may play an important role in the occurrence and development of uterine myoma by affecting the expression of ER 伪 in uterine myoma, and microRNA-18a may be a new target for the treatment of uterine myoma.
【作者单位】： 电子科技大学附属医院四川省人民医院妇产科;电子科技大学附属医院四川省人民医院药学部;
【基金】：四川省卫生厅基金资助项目(100491,120111) 四川省科技厅基金资助项目(2014FZ0103,2015JQO027,015ZR0160) 四川省人民医院青年基金资助项目(30305030606,30305030859)
【分类号】：R737.33
，
本文编号：2499124
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