胎盘凋亡及细胞转运与新生儿PBMC HBsAg关系
发布时间：2019-06-17 09:52
【摘要】：目的探讨胎盘凋亡及外周血单个核细胞(PBMC)母-胎转运与HBs Ag阳性孕妇新生儿PBMC HBs Ag的关系。方法用等位基因特异性PCR(As-PCR)筛选母儿信息病例对,用原位末端杂交法检测HBs Ag阳性孕妇足月胎盘凋亡指数,用免疫荧光双标法检测新生儿PBMC涂片上的HBs Ag和谷胱甘肽S转移酶(GST);采用SPSS 16.0软件进行分析。结果 86例新生儿PBMC中20例(23.26%)HBs Ag阳性,31例(36.05%)GST阳性,HBs Ag、GST共存者13例(15.12%);发生PBMC母-胎转运组的胎盘凋亡指数与未发生转运组的胎盘凋亡指数差异有统计学意义(t'=2.38,P=0.02),且胎盘滋养层细胞凋亡率高与PBMC转运相关(t=2.75,P=0.01);新生儿PBMC HBs Ag阳性组的胎盘细胞凋亡指数与阴性组的胎盘细胞凋亡指数差异无统计学意义(t=0.34,P=0.74);PBMC转运与新生儿PBMC HBs Ag阳性有关(χ2=9.48,P=0.00),发生PBMC转运的新生儿发生PBMC HBs Ag阳性的危险性是未发生PBMC转运新生儿的4.95倍(OR=4.95,95%CI=1.70~14.39)。结论妊晚期胎盘细胞凋亡增加有利于PBMC母-胎转运,感染HBV的PBMC可能通过胎盘进入胎儿血循环进而导致新生儿HBV感染。
[Abstract]:Objective to investigate the relationship between placental apoptosis and maternal-fetal transport of (PBMC) in peripheral blood mononuclear cells (PBMCs) and neonatal PBMC HBs Ag in HBs Ag positive pregnant women. Methods the maternal and infant information cases were screened by allele specific PCR (As-PCR). The apoptosis index of term placenta of HBs Ag positive pregnant women was detected by in situ end hybridization, and the HBs Ag and glutathione S transferase (GST); on neonatal PBMC smears were detected by immunofluorescence double standard method. SPSS 16.0 software was used to analyze the apoptosis index of term placenta. Results in 86 neonates with PBMC, 20 cases (23.26%) were) HBs Ag positive, 31 cases (36.05%) were GST positive, 13 cases (15.12%) were HBs Ag,GST, the placental apoptosis index of PBMC mother-fetal transport group was significantly different from that of non-transport group (t 鈮，
本文编号：2500903
[Abstract]:Objective to investigate the relationship between placental apoptosis and maternal-fetal transport of (PBMC) in peripheral blood mononuclear cells (PBMCs) and neonatal PBMC HBs Ag in HBs Ag positive pregnant women. Methods the maternal and infant information cases were screened by allele specific PCR (As-PCR). The apoptosis index of term placenta of HBs Ag positive pregnant women was detected by in situ end hybridization, and the HBs Ag and glutathione S transferase (GST); on neonatal PBMC smears were detected by immunofluorescence double standard method. SPSS 16.0 software was used to analyze the apoptosis index of term placenta. Results in 86 neonates with PBMC, 20 cases (23.26%) were) HBs Ag positive, 31 cases (36.05%) were GST positive, 13 cases (15.12%) were HBs Ag,GST, the placental apoptosis index of PBMC mother-fetal transport group was significantly different from that of non-transport group (t 鈮，
本文编号：2500903
本文链接：https://www.wllwen.com/yixuelunwen/fuchankeerkelunwen/2500903.html
最近更新
教材专著
