胰高血糖素样肽-1对高脂饲养大鼠卵巢生物钟节律的影响

发布时间：2019-06-18 09:57

【摘要】：目的观察胰高血糖素样肽-1(GLP-1)对高脂饲养大鼠受损卵巢生物钟的治疗作用。方法 Wistar大鼠随机分为普通饲养组、高脂饲养组及高脂饲养+GLP-1干预组;8周后测空腹血糖、胰岛素、胆固醇、甘油三酯、雌二醇、卵泡刺激素、黄体生成素的水平;实时定量PCR检测卵巢相关基因及生物钟基因的表达。结果 GLP-1对高脂饲养大鼠血清生化指标产生一定影响。与普通饲养组相比,高脂饲养组大鼠始基卵泡募集因子抗苗勒氏管激素(AMH)、生长分化因子-9(GDF-9)的表达水平降低(P0.05),而高脂饲养GLP-1干预组AMH、GDF-9的mRNA的表达明显改善。此外,与普通饲养组相比,高脂饲养组大鼠卵巢生物钟相关基因的表达具有明显变化,而GLP-1可改善高脂饲养对卵巢生物钟相关基因表达的影响。结论 GLP-1对高脂饲养所致的卵巢生物钟受损具有改善作用。
[Abstract]:Objective To observe the effect of glucagon-like peptide-1 (GLP-1) on the biological clock of rats with high-fat feeding. Methods Wistar rats were randomly divided into general feeding group, high-fat feeding group and high-fat feeding + GLP-1 intervention group. The levels of fasting blood glucose, insulin, cholesterol, triglyceride, estradiol, follicle stimulating hormone and luteinizing hormone were measured after 8 weeks. The expression of the ovary-related gene and the biological clock gene was detected by real-time quantitative PCR. Results GLP-1 had a certain effect on the serum biochemical indexes of high-fat-fed rats. Compared with the normal feeding group, the expression level of the growth differentiation factor-9 (GDF-9) and the expression level of the growth differentiation factor-9 (GDF-9) decreased (P0.05), while the expression of the mRNA of AMH and GDF-9 in the high-fat-fed GLP-1 intervention group was significantly improved. In addition, in that high-fat-fed group, the expression of the related gene of the ovarian biological clock in the high-fat-fed group has a significant change compared with the normal feeding group, and the GLP-1 can improve the effect of high-fat feeding on the expression of the ovarian clock-related genes. Conclusion GLP-1 can improve the biological clock of the ovary caused by high-fat feeding.
【作者单位】：山东大学附属生殖医院妇科;
【分类号】：R711.6

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