自噬在子宫肌瘤中作用研究
发布时间：2019-07-13 22:06
【摘要】：目的研究子宫肌瘤细胞中自噬的程度,探讨自噬对子宫肌瘤的影响。方法随机选取2013年7月至2014年6月该院因子宫肌瘤住院的患者20例,取子宫肌瘤组织和同一病例正常子宫平滑肌。其中各取10例组织消化为细胞,利用单丹黄酰尸胺(MDC)染色后检测荧光度,检测自噬囊泡数量。另取10例组织提取蛋白,利用Western blot检测自噬相关蛋白Ⅱ型微管相关蛋白轻链3(LC3-Ⅱ)、Beclin-1和P62表达水平。结果肌瘤细胞中自噬囊泡荧光度明显低于正常子宫平滑肌细胞(P0.05),肌瘤细胞中LC3-Ⅱ、Beclin-1和P62蛋白水平也明显低于正常子宫平滑肌细胞(P0.05)。结论子宫肌瘤细胞中自噬水平明显降低,自噬抑制可能为子宫肌瘤发生的原因之一。
[Abstract]:Objective to study the degree of autophagy in uterine myoma cells and to explore the effect of autophagy on uterine myoma. Methods from July 2013 to June 2014, 20 patients with uterine myoma were randomly selected. Uterine myoma tissue and normal uterine smooth muscle were taken. Among them, 10 cases of tissue were digested as cells, and the fluorescence was detected by (MDC) staining, and the number of autophagy vesicles was measured. The expression of autophagy-associated protein type 2 microtubule associated protein light chain 3 (LC3- 鈪，
本文编号：2514322
[Abstract]:Objective to study the degree of autophagy in uterine myoma cells and to explore the effect of autophagy on uterine myoma. Methods from July 2013 to June 2014, 20 patients with uterine myoma were randomly selected. Uterine myoma tissue and normal uterine smooth muscle were taken. Among them, 10 cases of tissue were digested as cells, and the fluorescence was detected by (MDC) staining, and the number of autophagy vesicles was measured. The expression of autophagy-associated protein type 2 microtubule associated protein light chain 3 (LC3- 鈪，
本文编号：2514322
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