辛二酰苯胺异羟肟酸联合紫杉醇对卵巢癌细胞OC3杀伤与自噬的影响

发布时间：2019-07-14 17:37

【摘要】：目的研究辛二酰苯胺异羟肟酸(suberoylanilide hydroxamic acid,SAHA)与紫杉醇(paclitaxel,PTX)联合应用对人卵巢癌细胞OC3杀伤及自噬的影响,探讨两药联合是否具有协同作用。方法利用SAHA和(或)PTX处理OC3细胞后,倒置显微镜下观察细胞形态;四甲基偶氮唑蓝(MTT)检测细胞的生长状态;AO/EB双染色观察细胞是否出现自噬泡,析因方差法与金式公式法分析联合用药效果。结果 SAHA和PTX单独或联合用药处理后,倒置显微镜下可见联合用药组细胞形态较单独用药组发生显著改变,细胞大部分出现折光率差和死亡的现象;MTT法检测细胞增殖结果表明,联合用药组较单独用药组显著抑制OC3细胞的增殖与存活(P0.05);析因方差分析显示,两药存在正交互作用,金式公式计算表明两药具有协同效应;AO/EB双染色发现各组细胞出现自噬现象,且联合用药组诱导自噬的发生率显著高于单独用药组。结论 SAHA联合PTX对人卵巢癌细胞OC3具有明显的协同杀伤作用,并可诱导其发生自噬。
[Abstract]:Objective to study the effect of octanodiamide hydroxamic acid (suberoylanilide hydroxamic acid,SAHA) combined with paclitaxel (paclitaxel,PTX) on the killing and autophagy of human ovarian cancer cell line OC3, and to explore whether the combination of the two drugs has synergistic effect. Methods OC3 cells were treated with SAHA and / or PTX, the morphology of OC3 cells was observed under inverted microscope, the growth state of OC3 cells was detected by (MTT), the autophagy was observed by AO/EB double staining, and the effect of combined administration was analyzed by factorial variance method and gold formula method. Results after treatment with SAHA and PTX alone or in combination, the morphology of cells in the combined group was significantly different from that in the treatment group alone, and most of the cells showed refractive index difference and death. The results of MTT assay showed that the proliferation of OC3 cells in the combined treatment group was significantly lower than that in the single treatment group (P 0.05). Factorial analysis of variance showed that there was positive interaction between the two drugs, and gold formula calculation showed that the two drugs had synergistic effect. AO/EB double staining showed autophagy in each group, and the incidence of autophagy induced in the combined drug group was significantly higher than that in the single drug group. Conclusion SAHA combined with PTX has obvious synergistic killing effect on human ovarian cancer cell line OC3 and can induce autophagy.
【作者单位】：空军总医院妇产科;军事医学科学院基础医学研究所细胞生物学研究室;大兴妇幼保健院妇产科;
【基金】：国家自然科学基金资助项目(31070996,31171084)
【分类号】：R737.31

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