筛选自人卵巢癌细胞系HO8910的干细胞生物学特性分析

发布时间：2019-08-09 17:45

【摘要】：目的探讨筛选自人卵巢癌细胞系HO8910干细胞的生物学特性。方法将前期筛选卵巢癌细胞系HO8910的干细胞在无血清培养基中进行传代培养,以HO8910细胞作为对照。体外观察两种细胞的球体形成能力;将两种细胞接种于含血清培养基,观察其分化能力,并检测干细胞相关标记物;采用药敏试验检测两种细胞对顺铂、多柔比星及米托蒽醌的敏感性;将无血清培养基中进行传代培养的两种细胞接种于NOD/SCID裸鼠,观察其体内成瘤情况。结果 HO8910细胞在无血清培养基中的球体形成能力明显低于卵巢癌细胞(P0.05)。卵巢癌干细胞分化前CD133、CD117阳性表达率明显高于分化后(P均0.05)。卵巢癌干细胞分化后ABCG2、Nanog、Oct4、BCRP、E-cadherin表达均低于分化前(P均0.05),与HO8910细胞分化前后上述指标比较差异均无统计学意义(P均0.05)。0.25、0.5μg/m L顺铂,0.5、1.5μmol/L多柔比星,0.05、0.25μg/m L米托蒽醌作用于卵巢癌干细胞、HO8910细胞48 h时,卵巢癌干细胞的相对活性均高于HO8910细胞(P均0.05)。裸鼠接种1×103个卵巢癌干细胞即可成瘤;随着干细胞接种数量的增加,成瘤比率逐渐增加,成瘤时间逐渐缩短(P均0.05)。结论筛选自人卵巢癌细胞系HO8910的卵巢癌干细胞具有自我更新和分化、体内成瘤、高表达干细胞基因以及对化疗耐药等特性,符合肿瘤干细胞的理论标准。
[Abstract]:Objective to investigate the biological characteristics of human ovarian cancer cell line HO8910. Methods the stem cells of ovarian cancer cell line HO8910 were subcultured in serum-free medium and HO8910 cells were used as control. The spherical formation ability of the two kinds of cells was observed in vitro, the differentiation ability of the two kinds of cells was observed, the differentiation ability was observed, and the related markers of stem cells were detected. The sensitivity of the two kinds of cells to DDP, doxorubicin and mitoxantrone was detected by drug sensitivity test. The two kinds of cells subcultured in serum-free medium were inoculated into NOD/SCID nude mice to observe their tumorigenesis in vivo. Results the globular formation ability of HO8910 cells in serum-free medium was significantly lower than that of ovarian cancer cells (P 0.05). The positive expression rate of CD133,CD117 in ovarian cancer stem cells before differentiation was significantly higher than that after differentiation. The expression of ABCG2,Nanog,Oct4,BCRP,E-cadherin in ovarian cancer stem cells after differentiation was lower than that before differentiation, but there was no significant difference compared with that before and after differentiation of HO8910 cells. 0.25 渭 g / mL DDP, 0.5 渭 mol / L doxorubicin, 0.05 渭 g / mL mitoxantrone and 0.25 渭 g / mL mitoxantrone on ovarian cancer stem cells for 48 h. The relative activity of ovarian cancer stem cells was higher than that of HO8910 cells. With the increase of the number of stem cells inoculated with 1 脳 103 ovarian cancer stem cells in nude mice, the tumorigenesis rate increased gradually and the tumorigenesis time decreased gradually (P 0.05). Conclusion the ovarian cancer stem cells screened from human ovarian cancer cell line HO8910 have the characteristics of self-renewal and differentiation, tumorigenesis in vivo, high expression of stem cell genes and resistance to chemotherapy, which is in accordance with the theoretical standard of tumor stem cells.
【作者单位】：徐州医学院;徐州医学院附属医院;
【分类号】：R737.31

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