碱性成纤维细胞生长因子基因修饰骨骼肌卫星细胞自体移植急性心肌梗死组织的血管新生

发布时间：2018-04-07 16:41

本文选题：卫星细胞　切入点：骨骼肌　出处：《中国组织工程研究》2017年09期

【摘要】：背景:前期研究发现,骨骼肌卫星胞移植能够诱导心肌梗死区新生血管形成,缩小梗死面积,改善其心功能,但整体效果并不太理想。目的:观察碱性成纤维细胞生长因子基因修饰骨骼肌卫星细胞在急性心肌梗死区的存活及对心肌梗死区血管新生的影响。方法:将18只新西兰大白兔随机分为3组,实验组、对照组结扎冠状动脉左前降支,构建急性心肌梗死动物模型;空白对照组只穿线,不结扎。造模成功即刻,实验组于局部梗死心肌内注射DAPI标记的碱性成纤维细胞生长因子基因修饰自体骨骼肌卫星细胞悬液50μL,对照组注射等量DAPI标记的自体骨骼肌卫星细胞。细胞移植4周后取标本,观察心肌梗死区骨骼肌卫星细胞存活及成纤维细胞生长因子基因表达情况,免疫组织化学染色检查心肌梗死区新生血管形成情况。结果与结论:(1)空白对照组未见DAPI标记的细胞,对照组及实验组缺血心肌区域均可见大量DAPI标记的骨骼肌卫星细胞,实验组还可见大量EGFP-碱性成纤维细胞生长因子融合蛋白绿色荧光表达;(2)实验组、对照组新生微血管密度多于空白对照组(P0.05),实验组新生微血管密度多于对照组(P0.05);(3)结果表明,碱性成纤维细胞生长因子修饰骨骼肌卫星细胞可在急性心肌梗死区存活,促进心肌梗死区血管新生。
[Abstract]:Background: previous studies have shown that skeletal muscle satellite cell transplantation can induce neovascularization, reduce infarct size and improve cardiac function in myocardial infarction area, but the overall effect is not very satisfactory.Aim: to investigate the survival and angiogenesis of basic fibroblast growth factor gene modified skeletal muscle satellite cells in acute myocardial infarction (AMI).Methods: eighteen New Zealand white rabbits were randomly divided into three groups: the experimental group, the control group ligated the left anterior descending coronary artery, and the blank control group only threaded and did not ligate the left anterior descending coronary artery.Immediately after the establishment of the model, the experimental group was injected with 50 渭 L DAPI labeled basic fibroblast growth factor gene modified autologous skeletal muscle satellite cell suspension, and the control group was injected with the same amount of DAPI labeled autologous skeletal muscle satellite cell.The survival of skeletal muscle satellite cells and the expression of fibroblast growth factor gene were observed after 4 weeks of cell transplantation. The neovascularization in myocardial infarction area was detected by immunohistochemical staining.Results and conclusion No DAPI labeled cells were found in the blank control group, and a large number of DAPI labeled skeletal muscle satellite cells were found in the ischemic myocardium of the control group and the experimental group.A large number of EGFP-basic fibroblast growth factor fusion protein was also found in the experimental group. The results showed that the neonate microvessel density in the control group was higher than that in the blank control group, and the neonate microvessel density in the experimental group was higher than that in the control group.Basic fibroblast growth factor modified skeletal muscle satellite cells can survive in acute myocardial infarction and promote angiogenesis.
【作者单位】：北京大学深圳医院心血管外科;
【基金】：深圳市科技计划项目(200404104)~~
【分类号】：R542.22

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