塞来昔布下调Apaf-1蛋白表达促进大鼠颅脑损伤后学习记忆功能恢复的研究

发布时间：2018-05-13 14:26

本文选题：脑损伤 + 环氧化酶-　；参考：《重庆医学》2017年13期

【摘要】：目的探讨塞来昔布对大鼠创伤性脑损伤后学习记忆功能和环氧化酶(COX-2)及凋亡蛋白酶活化因子-1(Apaf-1)蛋白表达的影响。方法将72只成年雄性Wistar大鼠等量分为对照组、假手术组、损伤组和治疗组,术后72h灌注取脑,应用免疫组化法和Western blot法分别检测COX-2及Apaf-1蛋白表达变化;术前5d和术后72h采用Morris水迷宫实验观察大鼠学习记忆功能。结果损伤组COX-2和Apaf-1蛋白表达明显高于其他组,治疗组与损伤组比较蛋白表达均下降(P0.05),但仍高于假手术组和对照组(P0.05);Morris水迷宫实验中,损伤组逃避潜伏期时间延长为4组之最(P0.05),治疗组较损伤组时间有所缩短(P0.05)。结论 COX-2抑制剂塞来昔布可下调COX-2和Apaf-1蛋白表达,抑制炎性反应、细胞凋亡,改善脑损伤后的学习、记忆障碍。
[Abstract]:Objective to investigate the effect of celecoxib on the learning and memory function and the expression of cyclooxygenase (COX-2) and apoptotic protease activator -1 (Apaf-1) protein after traumatic brain injury in rats. Methods 72 adult male Wistar rats were divided into control group, sham operation group, injury group and treatment group, 72h perfusion was used to take brain after operation, immunohistochemistry and We were used. The expression of COX-2 and Apaf-1 protein was detected by stern blot, and the learning and memory function of rats was observed by Morris water maze before 5D and 72h after operation. The results showed that the expression of COX-2 and Apaf-1 protein in the injured group was significantly higher than that in the other groups, and the protein expression in the treatment group was lower than that in the injured group (P0.05), but it was still higher than that of the sham operation group and the control group (P0.05). In the Morris water maze experiment, the escape latency of the injury group was prolonged in 4 groups (P0.05), and the time of the treatment group was shorter than that of the injured group (P0.05). Conclusion the COX-2 Inhibitor Celecoxib could reduce the expression of COX-2 and Apaf-1 protein, inhibit the inflammatory reaction, cell apoptosis, improve the learning and memory impairment after brain injury.

【作者单位】：河北医科大学附属邢台人民医院;邢台医学高等专科学校;华北理工大学医学院;
【基金】：河北省自然科学基金资助项目(C2004000689) 河北省博士基金资助项目(05547008D-4) 河北省科学技术与社会发展计划项目(04276135)
【分类号】：R651.15

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