复方丹参注射液对感染休克大鼠脑损伤时NO、bcl-2及S100B的影响

发布时间：2018-05-13 14:47

本文选题：复方丹参注射液 + NO　；参考：《南昌大学》2013年硕士论文

【摘要】：目的：探讨复方丹参注射液对感染性休克大鼠脑损伤时NO、bcl-2及S100B的影响。方法：选2周龄健康Wistar大鼠40只，随机分为四个组，每组10只。正常对照组即颈动脉插管监测血压，在其他组给予药物时给予生理盐水替代；感染性休克组1（6h）及感染性休克组2（12h）即颈动脉插管监测血压，通过尾静脉给予脂多糖（5mg/kg)复制感染性休克模型，血压降为原来的2/3，伴躁动、皮肤发绀等现象说明造模成功；复方丹参注射液治疗组即造模成功后通过腹腔注射给予复方丹参注射（5ml/kg）。除感染性休克组2于造模成功12h取材外，其余三组均于造模后6小时取材，用硝酸还原法检测四组鼠脑组织中NO的含量，免疫组化法检测四组鼠海马组织中bcl-2的表达及ELISA法检测四组鼠静脉血S100B。结果： 1.脑组织NO的含量：感染性休克组1（6h）NO含量高于正常组（P0.05），与感染性休克组1相比，治疗组NO含量明显降低（P0.05），但是感染性休克组2（12h）NO含量高于感染性休克组1（6h）（P0.05）。 2.海马组织bcl-2的表达：正常组、感染性休克组1（6h）及治疗组三组组间比较有统计学差异（P0.05）。与正常组比较，，感染性休克组1及治疗组bcl-2的表达均明显增多（P0.05），且治疗组较感染性休克组1bcl-2表达增多（P0.05），但是感染性休克组2（12h）bcl-2表达低于感染性休克组1（6h）（P0.05）。 3.血清S100B的含量：感染性休克组1（6h）S100B含量高于正常组（P0.05），与感染性休克组1相比，治疗组S100B含量明显降低（P0.05），但是感染性休克组2（12h）S100B含量高于感染性休克组1（6h）P0.05）。 4.脑组织病理学改变：正常对照组：脑组织切片细胞层次清晰，结构完整，神经细胞胞质丰富，核质细，核仁清楚；感染性休克组1及感染性休克组2：正常组织结构消失或结构欠清,间质水肿,神经细胞排列紊乱，结构模糊,部分细胞坏死；复方丹参治疗组：正常组织结构尚存在,间质水肿轻,神经细胞排列规则，细胞核固缩少，结构较完整。 5.脑组织NO含量与海马组织bcl-2相关性分析：脑组织NO含量与海马组织bcl-2之间存在负相关（r=-0.928，P 0.05）。 6.脑组织NO含量与血清S100B含量相关性分析：脑组织NO含量与血清S100B之间存在正相关（r=0.877，P0.05）。结论： 1.NO参与了感染性休克脑损伤的过程，可能与其下调bcl-2有关。 2.NO和S100B共同参与了感染性休克脑损伤的过程。 3.S100B的表达可以反映感染性休克时脑损伤的程度。 4.感染性休克时，复方丹参注射液对脑组织具有保护作用，可能与抑制NO的生成，上调bcl-2的表达有关。
[Abstract]:Objective: to investigate the effect of compound salvia miltiorrhiza injection on NO-BCL 2 and S 100B during brain injury in rats with septic shock. Methods: 40 2-week healthy Wistar rats were randomly divided into four groups with 10 rats in each group. The blood pressure was monitored by carotid artery intubation in the normal control group, and replaced by normal saline in the other groups; in the septic shock group, the blood pressure was monitored by carotid artery catheterization for 1 to 6 h) and the septic shock group for 21 ~ 12 h), the blood pressure was monitored by carotid artery intubation. The septic shock model was established by injecting lipopolysaccharide (LPS) 5 mg / kg via tail vein. The blood pressure dropped to 2 / 3 of the original, accompanied by restlessness and cyanosis. The treatment group of compound salvia miltiorrhiza injection was given 5 ml / kg of compound salvia miltiorrhiza injection by intraperitoneal injection. Except for the septic shock group (group 2), the other three groups were collected at 6 hours after modeling, and the contents of no in the brain tissue of the four groups were detected by nitric acid reduction method. Immunohistochemical method was used to detect the expression of bcl-2 in hippocampal tissue of the four groups and ELISA method to detect S100B in the venous blood of the four groups. Results: 1. No content in brain tissue: 1(6h)NO content in septic shock group was higher than that in normal group. Compared with septic shock group 1, no content in treatment group was significantly lower than that in septic shock group, but 2(12h)NO content in septic shock group was higher than that in septic shock group at 16 h. 2. The expression of bcl-2 in hippocampal tissue: normal group, septic shock group (1: 6 h) and treatment group (P 0.05). Compared with the normal group, the expression of bcl-2 in the septic shock group and the treatment group was significantly higher than that in the septic shock group, and the expression of 1bcl-2 in the treatment group was higher than that in the septic shock group, but the 2(12h)bcl-2 expression in the septic shock group was lower than that in the septic shock group at 16 h. 3. The content of serum S100B: the content of 1(6h)S100B in septic shock group was higher than that in normal group (P 0.05). Compared with septic shock group 1, the content of S100B in treatment group was significantly lower than that in septic shock group, but the content of 2(12h)S100B in septic shock group was higher than that in septic shock group at 1 h after shock. 4. Brain histopathological changes: normal control group: the level of brain tissue slice cells is clear, the structure is complete, the nerve cell cytoplasm is abundant, the nucleus is fine, the nucleolus is clear; Septic shock group 1 and septic shock group 2: normal tissue structure disappeared or not clear, interstitial edema, nerve cell arrangement disorder, fuzzy structure, partial cell necrosis, compound salvia miltiorrhiza treatment group: normal tissue structure still existed, The interstitial edema is light, the nerve cells are arranged regularly, the nucleus is less pyknosis and the structure is relatively complete. 5. The correlation between no content in brain tissue and bcl-2 in hippocampus: there was a negative correlation between no content and bcl-2 in hippocampus (P 0.05). 6. The correlation between no content in brain tissue and serum S100B content: there was a positive correlation between no content in brain tissue and serum S100B content. Conclusion: 1.NO is involved in the process of septic shock brain injury and may be related to its down-regulation of bcl-2. 2.NO and S100B are involved in the process of septic shock brain injury. The expression of 3.S100B can reflect the degree of brain injury in septic shock. 4. In septic shock, compound salvia miltiorrhiza injection has protective effect on brain tissue, which may be related to inhibition of no production and up-regulation of bcl-2 expression.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R459.7

【参考文献】